Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse wild type R1 and cells expressing Pax4 following induction of pacreatic differentitation

ABSTRACT: This experiment is part of the FunGenES project (FunGenES - "Functional Genomics in Embryonic Stem Cells" partially funded by the 6th Framework Programme of the European Union, http://www.fungenes.org). The experiment was conducted at the Leibniz Institute of Plant Genetics and Crop Plant Research (IPK, Gatersleben, Germany) The goal of the experiment is to optimize existing differentiation protocols for endoderm differentiation of ES cells to efficiently generate optimal levels of pancreatic cell types. To characterize potential pancreatic progenitors during the process of induced pancreatic differentiation, wild type R1 cells and R1 cells stably expressing the pancreatic control gene Pax4, RNA samples of undifferentiated cells, pancreatic precursor and differentiated cells were prepared.

ORGANISM(S): Mus musculus

SUBMITTER: Oliver Hummel 

PROVIDER: E-TABM-493 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Characterization of mouse embryonic stem cell differentiation into the pancreatic lineage in vitro by transcriptional profiling, quantitative RT-PCR and immunocytochemistry.

Rolletschek Alexandra A   Schroeder Insa S IS   Schulz Herbert H   Hummel Oliver O   Huebner Norbert N   Wobus Anna M AM  

The International journal of developmental biology 20100101 1

We have previously shown that mouse embryonic stem (ES) cells differentiate into insulin-positive cells via multi-lineage progenitors. Here, we used Affymetrix chips and quantitative RT-PCR analysis to determine transcriptional profiles of undifferentiated wildtype (wt) and Pax4 expressing (Pax4+) ES cells and differentiated cells of committed progenitor and advanced stages. From undifferentiated to the committed stage, 237 (wt) and 263 (Pax4+) transcripts were 5- or more-fold up-regulated, wher  ...[more]

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