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MicroRNA-Based Classification of Hepatocellular Carcinoma and Oncogenic Role of miR-517a

ABSTRACT: Hepatocellular carcinoma (HCC) is a complex and heterogeneous tumor due to activation of multiple cellular pathways and molecular alterations. Herein, we report the first molecular classification of 89 HCC based on the expression of 358 microRNAs and integrative genomic analysis. Three main subclasses of HCC were identified : two of them were associated with beta-catenin mutations or aggressive phenotype. A subset of the subclass of aggressive tumors (8/89, 9%) showed overexpression of a cluster of microRNAs located on chr19q13.41 (C19MC locus. We showed that miR 517a, representing C19MC, promoted cell proliferation, migration and invasion in vitro and induced the development of aggressive tumors in vivo suggesting its role as a novel oncogenic driver in HCC. This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

ORGANISM(S): Homo sapiens

SUBMITTER: Yujin Hoshida

PROVIDER: E-GEOD-20596 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Induction of hepatocellular carcinoma by in vivo gene targeting.

Wang Pei-Rong PR Xu Mei M Toffanin Sara S Li Yi Y Llovet Josep M JM Russell David W DW

Proceedings of the National Academy of Sciences of the United States of America 20120625 28

The distinct phenotypic and prognostic subclasses of human hepatocellular carcinoma (HCC) are difficult to reproduce in animal experiments. Here we have used in vivo gene targeting to insert an enhancer-promoter element at an imprinted chromosome 12 locus in mice, thereby converting ∼1 in 20,000 normal hepatocytes into a focus of HCC with a single genetic modification. A 300-kb chromosomal domain containing multiple mRNAs, snoRNAs, and microRNAs was activated surrounding the integration site. An ...[more]

PMID: 22733778

Publication: 1/2

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