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Expression data from WT and TCF-1-deficient memory CD8 T cells

ABSTRACT: TCF-1 is an HMG family transcription factor which is known to be activated by the canonical Wnt signaling pathway and modulated by other signals such as those derived from T cell receptor. We found that during CD8 T cell responses, TCF-1 deficiency impaired long-term maintenance of antigen-specific memory CD8 T cells. We used microarrays to detect gene expression changes in memory CD8 T cells caused by TCF-1 deficiency. Host mice that received WT or TCF-1-deficient OT-I CD8 T cells were infected with attenuated Listeria monocytogenes expressing Ova peptide. In memory phase (i.e., more than 80 days post infection), antigen-specific T cells were ioslated by cell sorting. RNA was extracted and hybridized to GeneChip Mouse GENE 1.0 ST arrays (Affymetrix).

ORGANISM(S): Mus musculus

SUBMITTER: Xinyuan Zhou

PROVIDER: E-GEOD-20754 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Differentiation and persistence of memory CD8(+) T cells depend on T cell factor 1.

Zhou Xinyuan X Yu Shuyang S Zhao Dong-Mei DM Harty John T JT Badovinac Vladimir P VP Xue Hai-Hui HH

Immunity 20100801 2

T cell factor 1 (TCF-1) is a transcription factor known to act downstream of the canonical Wnt pathway and is essential for normal T cell development. However, its physiological roles in mature CD8(+) T cell responses are unknown. Here we showed that TCF-1 deficiency limited proliferation of CD8(+) effector T cells and impaired their differentiation toward a central memory phenotype. Moreover, TCF-1-deficient memory CD8(+) T cells were progressively lost over time, exhibiting reduced expression ...[more]

PMID: 20727791

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Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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