Unknown,Transcriptomics,Genomics,Proteomics

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Whole-genome microarray analysis of primary, secondary, tertiary and quaternary memory CD8 T cells


ABSTRACT: The transcriptome of naive OT-I T cells was compared to memory CD8 T cells after 1, 2, 3, or 4 infection with ovalbumin expressing Listeria monocytogenes (LM-OVA). Naive Thy1.1 OT-I T cells were adoptively transferred into Thy1.2 naive hosts prior to infection with LM-OVA. The resulting memory CD8 T cell population was again adoptively transferred into naive hosts and the recipient mice were again infected with LM-OVA. The adoptive transfer was repeated up to four times to generate memory CD8 T cells with up to four consecutive antigen stimulations. Three individual mice were analyzed for each group. For quaternary memory CD8 T cells, spleens from two to three mice were pooled for each sample. Naive OT-I T cells served as control samples. http://dx.doi.org/10.1016/j.immuni.2010.06.014

ORGANISM(S): Mus musculus

SUBMITTER: Hai-Hui Xue 

PROVIDER: E-GEOD-21360 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Repetitive antigen stimulation induces stepwise transcriptome diversification but preserves a core signature of memory CD8(+) T cell differentiation.

Wirth Thomas C TC   Xue Hai-Hui HH   Rai Deepa D   Sabel Jaime T JT   Bair Tom T   Harty John T JT   Badovinac Vladimir P VP  

Immunity 20100708 1


Repetitive antigen stimulation by prime-boost vaccination or pathogen reencounter increases memory CD8(+) T cell numbers, but the impact on memory CD8(+) T cell differentiation is unknown. Here we showed that repetitive antigen stimulations induced accumulation of memory CD8(+) T cells with uniform effector memory characteristics. However, genome-wide microarray analyses revealed that each additional antigen challenge resulted in the differential regulation of several hundred new genes in the en  ...[more]

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