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Genome-wide Analyses of Transcription Factor GATA3-Mediated Gene Regulation in Distinct T Cell Types


ABSTRACT: We report genome-wide characterization of GATA3 binding sites in eleven well-defined developmental and effector cell types of the T lymphocyte lineage. By utilizing a conditional allele of GATA3, we investigated the impact of GATA3 expression on the mRNA expression patterns in several of these cell types. Correlation of GATA3 binding with gene expression changes indicates that GATA3 regulates a large number of stage- and cell-specific genes involved in multiple signaling and transcriptional pathways critical for T cell differentiation and immune responses. 1) RNA-Seq: Examination of RNA transcript levels in 6 cell types in wild-type and Gata3-knockout cells. 2) ChIP-Seq: Examination of GATA3 binding sites in 11 cell types. 3) Methyl-Seq: Examination of histone modification levels in DP cells from wild-type and Gata3-knockout mice.

ORGANISM(S): Mus musculus

SUBMITTER: Brian Abraham 

PROVIDER: E-GEOD-20898 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide analyses of transcription factor GATA3-mediated gene regulation in distinct T cell types.

Wei Gang G   Abraham Brian J BJ   Yagi Ryoji R   Jothi Raja R   Cui Kairong K   Sharma Suveena S   Narlikar Leelavati L   Northrup Daniel L DL   Tang Qingsong Q   Paul William E WE   Zhu Jinfang J   Zhao Keji K  

Immunity 20110801 2


The transcription factor GATA3 plays an essential role during T cell development and T helper 2 (Th2) cell differentiation. To understand GATA3-mediated gene regulation, we identified genome-wide GATA3 binding sites in ten well-defined developmental and effector T lymphocyte lineages. In the thymus, GATA3 directly regulated many critical factors, including Th-POK, Notch1, and T cell receptor subunits. In the periphery, GATA3 induced a large number of Th2 cell-specific as well as Th2 cell-nonspec  ...[more]

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