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Characterization of miRNA transcriptome of skeletal muscle from broiler and layer chicken lines by deep sequencing

ABSTRACT: In the current study, we expanded our previous work to identify miRNAs implicated in the myogenesis regulation through the comparison of miRNAs transcriptome in skeletal muscle tissues between broilers and layers. To address that, we firstly performed Solexa deep sequencing to profile miRNAs expressed in chicken skeletal muscle tissues. Sequence tags analyses not only enable us to report a group of highly abundant known miRNAs expressed in skeletal muscles but most importantly to identify novel putative chicken miRNAs from skeletal muscle tissue. Further miRNA transcriptome comparison and real-time RT-PCR validation experiments revealed seveal differentially expressed miRNAs between broilers and layers. Examination of miRNA transcriptome in skeletal muscle of two kinds of chickens

ORGANISM(S): Gallus gallus

SUBMITTER: Tingting Li

PROVIDER: E-GEOD-20942 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A systematic analysis of the skeletal muscle miRNA transcriptome of chicken varieties with divergent skeletal muscle growth identifies novel miRNAs and differentially expressed miRNAs.

Li Tingting T Wu Rimao R Zhang Yong Y Zhu Dahai D

BMC genomics 20110413

<h4>Background</h4>Functional studies have demonstrated that microRNAs (miRNAs or miRs) play critical roles in a wide spectrum of biological processes including development and disease pathogenesis. To investigate the functional roles that miRNAs play during chicken skeletal muscle development, the miRNA transcriptomes of skeletal muscles from broiler and layer chickens were profiled using Solexa deep sequencing.<h4>Results</h4>Some miRNAs have multiple isoforms and several miRNAs* are present a ...[more]

PMID: 21486491

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