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Whole genome bisulfite-seq of two healthy males.

ABSTRACT: Bisulfite-seq data sets were generated for peripheral blood lymphocyte (PBL) and hair follicle (HF) DNA from each of two healthy males. Examination of genome-wide CpG methylation two tissues (hair follicle and peripheral blood lymphocyte) from 2 healthy male individuals.

ORGANISM(S): Homo sapiens

SUBMITTER: Govindarajan Kunde Ramamoorthy

PROVIDER: E-GEOD-44806 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Comparison and quantitative verification of mapping algorithms for whole-genome bisulfite sequencing.

Kunde-Ramamoorthy Govindarajan G Coarfa Cristian C Laritsky Eleonora E Kessler Noah J NJ Harris R Alan RA Xu Mingchu M Chen Rui R Shen Lanlan L Milosavljevic Aleksandar A Waterland Robert A RA

Nucleic acids research 20140103 6

Coupling bisulfite conversion with next-generation sequencing (Bisulfite-seq) enables genome-wide measurement of DNA methylation, but poses unique challenges for mapping. However, despite a proliferation of Bisulfite-seq mapping tools, no systematic comparison of their genomic coverage and quantitative accuracy has been reported. We sequenced bisulfite-converted DNA from two tissues from each of two healthy human adults and systematically compared five widely used Bisulfite-seq mapping algorithm ...[more]

PMID: 24391148

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