Comparison of polyribosomal to total transcript profiles
Ontology highlight
ABSTRACT:
ORGANISM(S): Arabidopsis thaliana
SUBMITTER: David Galbraith
PROVIDER: E-GEOD-2132 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Immunopurification of polyribosomal complexes of Arabidopsis for global analysis of gene expression.
Zanetti María Eugenia ME Chang Ing-Feng IF Gong Fangcheng F Galbraith David W DW Bailey-Serres Julia J
Plant physiology 20050601 2
Immunoaffinity purification of polyribosomes (polysomes) from crude leaf extracts of Arabidopsis (Arabidopsis thaliana) was achieved with transgenic genotypes that overexpress a translational fusion of a ribosomal protein (RP) with a His(6)-FLAG dual epitope tag. In plants with a cauliflower mosaic virus 35S:HF-RPL18 transgene immunopurification with anti-FLAG agarose beads yielded 60-Svedberg ribosomal subunits, intact 80-Svedberg monosomes and polysomes. Sucrose density gradient fractionation ...[more]
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