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The gene regulatory cascade linking proneural specification with differentiation in Drosophila sensory neurons


ABSTRACT: A challenge in systems biology is to understand the gene regulatory networks that connect early cellular specification to terminal differentiation of specific cell types. In neurogenesis, neural specification has been well studied, but the link between the proneural transcriptional regulators of specification and the genes that must be activated to construct differentiated neurons is obscure. High resolution temporal profiling of gene expression reveals the events downstream of Atonal (Ato) proneural gene function in Drosophila sensory neurons. Unexpectedly, many differentiation genes are activated soon after specification, even before cell cycle exit and overt neuronal differentiation. Prominent among them are genes required for construction of the ciliary dendrite. Ato activates differentiation both directly and indirectly via several intermediate transcriptional regulators, including Rfx and a new Forkhead family factor. Our analysis of these factors and their regulation provides insight into how proneural factors regulate neuronal subtype differentiation. Investigating the molecular mechanisms of peripheral nervous sytem development in Drosophila melanogaster. Affymetrix Drosophila version 2.0 chips used to measure gene expression from GFP+ and GFP- cells from embryos expressing GFP under the control of the atonal gene enhancer in both wild type and mutant embryos. Data generated for three developmental time-points in quadruplicate.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Ian Simpson 

PROVIDER: E-GEOD-21520 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The gene regulatory cascade linking proneural specification with differentiation in Drosophila sensory neurons.

Cachero Sebastián S   Simpson T Ian TI   Zur Lage Petra I PI   Ma Lina L   Newton Fay G FG   Holohan Eimear E EE   Armstrong J Douglas JD   Jarman Andrew P AP  

PLoS biology 20110104 1


In neurogenesis, neural cell fate specification is generally triggered by proneural transcription factors. Whilst the role of proneural factors in fate specification is well studied, the link between neural specification and the cellular pathways that ultimately must be activated to construct specialised neurons is usually obscure. High-resolution temporal profiling of gene expression reveals the events downstream of atonal proneural gene function during the development of Drosophila chordotonal  ...[more]

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