Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from testis of day-7 Sohlh1 knock-out mice

ABSTRACT: Sohlh1 and Sohlh2 encode a germ cell-specific basic helix-loop-helix transcriptional regulator critical in spermatogonial differentiation. Seven-day-old Sohlh1 or Sohlh2 knockout and wild-type testes were arrayed on the Affy 430 2.0 platform. The following mice were analyzed at postnatal day 7: wild-type, Sohlh1-/-, and Sphlh2-/-. 4 samples/group.

ORGANISM(S): Mus musculus

SUBMITTER: Aleksandar Rajkovic

PROVIDER: E-GEOD-21525 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Sohlh1 is essential for spermatogonial differentiation.

Ballow D D Meistrich M L ML Matzuk M M Rajkovic A A

Developmental biology 20060329 1

Spermatogonia are adult germline stem cells that can both self-renew and differentiate into spermatocytes. Little is known about factors necessary for spermatogonial differentiation. We identified a novel germ-cell-specific transcription factor that we named Sohlh1 (testis and ovary expressed basic helix-loop-helix (bHLH) transcription factor). In males, Sohlh1 is preferentially expressed in prespermatogonia and Type A spermatogonia. Loss of Sohlh1 causes infertility by disrupting spermatogonial ...[more]

PMID: 16564520

Similar Datasets

Transcriptional changes in Sohlh1 knockout and Sohlh2 knockout mouse newborn ovaries

Project description:Sohlh1 and Sohlh2 are germ cell-specific basic helix-loop-helix transcription factors critical in early folliculogenesis. We discovered that Sohlh1 and Sohlh2 knockout females lose oocytes after birth and few remains by postnatal day 14. Here, we show that many genes preferentially expressed in the oocytes are misregulated by Sohlh1 and/or Sohlh2 deficiency. Total RNA samples isolated from wildtype, Sohlh1 KO and Sohlh2 KO mouse newborn ovaries were arrayed on the Illumina BeadChip MouseWG-6 2.0 arrays. We have 4 replicates for wildtype and Sohlh1 KO and 3 replicates for Sohlh2 KO.

2010-10-22 | E-GEOD-24816 | biostudies-arrayexpress

Transcriptional changes in Sohlh1 knockout and Sohlh2 knockout mouse newborn ovaries

2010-10-22 | GSE24816 | GEO

Transcriptional changes in Sohlh1/Sohlh2 double knockout newborn mouse ovaries

Project description:Sohlh1 and Sohlh2 are germ cell-specific basic helix-loop-helix transcription factors critical in early folliculogenesis. Differential genes expression by both Sohlh1 and Sohlh2 deficiency in mouse newborn ovaries was accessed using microarray. RNA samples from Sohlh1/ Sohlh2 double knockout and wild-type newborn ovaries were arrayed on the Illumina beadchip mouse WG-6 2.0.

2010-10-22 | GSE24815 | GEO

Expression data from testis of day-7 Sohlh1 knock-out mice

Project description:Sohlh1 and Sohlh2 encode a germ cell-specific basic helix-loop-helix transcriptional regulator critical in spermatogonial differentiation. Seven-day-old Sohlh1 or Sohlh2 knockout and wild-type testes were arrayed on the Affy 430 2.0 platform.

2010-04-27 | GSE21525 | GEO

Transcriptional changes in Sohlh1/Sohlh2 double knockout newborn mouse ovaries

2010-10-22 | E-GEOD-24815 | biostudies-arrayexpress

Regulation of early folliculogenesis by Sohlh1 and Sohlh2 in mouse ovary

Project description:This SuperSeries is composed of the following subset Series: GSE24815: Transcriptional changes in Sohlh1/Sohlh2 double knockout mouse newborn ovaries GSE24816: Transcriptional changes in Sohlh1 knockout and Sohlh2 knockout mouse newborn ovaries Refer to individual Series

2010-10-22 | E-GEOD-24817 | biostudies-arrayexpress

FIGLA, LHX8 and SOHLH1 transcription factor networks regulate mouse oocyte growth and differentiation

Project description:Germ-cell transcription factors control gene networks that regulate primordial follicle formation and oocyte differentiation during early, postnatal mouse oogenesis. Taking advantage of gene-edited mice lacking transcription factors expressed in female germ cells, we analyzed global gene expression profiles in perinatal ovaries from wildtype, FiglaNull, Lhx8Null and SohlhNull mice. Figla deficiency dysregulates expression of meiosis-related genes (e.g., Sycp3, Rad51 and Msy2) and a variety of genes (e.g., Nobox, Lhx8, Taf4b, Sohlh1, Sohlh2 and Gdf9) associated with oocyte growth and differentiation. The absence of FIGLA significantly impedes meiotic progression, causes DNA damage and results in oocyte apoptosis. Moreover, we find that FIGLA and other transcriptional regulators (e.g., NOBOX, LHX8, SOHLH1 and SOHLH2) are co-expressed in the same subset of germ cells in perinatal ovaries and Figla ablation dramatically disrupts KIT, NOBOX, LHX8, SOHLH1 and SOHLH2 expression. In addition, not only do FIGLA, SOHLH1 and LHX8 cross-regulate each other, they also cooperate by direct interaction with each during early oocyte development and share downstream gene targets. Thus, our findings substantiate a major role for FIGLA, LHX8 and SOHLH1 as multifunctional regulators of networks necessary for oocyte maintenance and differentiation during early folliculogenesis.

2019-11-06 | GSE139966 | GEO