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Oscillating gene expression determines competence for periodic branching in the Arabidopsis root

ABSTRACT: The Oscillation Zone (OZ) of unsynchronized roots was dissected and divided into an upper (OZ2) and lower (OZ1) half . Samples were ordered according to DR5:GUS expression as analyzed by real time PCR to find periodic expression patterns Seedlings were grown for 5 days. 39 individual roots were dissected for RNA extraction. These microarray samples profile a specific region of the root tip designated as the Oscillation zone. Arrangement of the samples in the following order: OZ1-1, OZ1-4, OZ1-23, OZ1-14, OZ1-17, OZ1-2, OZ1-6, OZ1-13, OZ1-15, OZ1-16, OZ1-10, OZ1-32, OZ1-3, OZ1-31, OZ1-22, OZ1-35, OZ1-19, OZ1-9, OZ1-33, OZ1-20, OZ2-4, OZ2-23, OZ2-14, OZ2-6, OZ2-13, OZ2-15, OZ2-10, OZ2-32, OZ2-22, OZ2-35, OZ2-19, OZ2-9, OZ2-20, OZ2-36, OZ2-39, OZ2-21, OZ2-40, OZ2-38, OZ2-1, was used to identify periodic expression patterns.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Miguel Moreno-Risueno

PROVIDER: E-GEOD-21611 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Oscillating gene expression determines competence for periodic Arabidopsis root branching.

Moreno-Risueno Miguel A MA Van Norman Jaimie M JM Moreno Antonio A Zhang Jingyuan J Ahnert Sebastian E SE Benfey Philip N PN

Science (New York, N.Y.) 20100901 5997

Plants and animals produce modular developmental units in a periodic fashion. In plants, lateral roots form as repeating units along the root primary axis; however, the developmental mechanism regulating this process is unknown. We found that cyclic expression pulses of a reporter gene mark the position of future lateral roots by establishing prebranch sites and that prebranch site production and root bending are periodic. Microarray and promoter-luciferase studies revealed two sets of genes osc ...[more]

PMID: 20829477

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