Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of time series of C.elegans mutants and wild type


ABSTRACT: This series of samples comprises multiple early embryonic time courses for C. elegans. Time courses consisting of 10 time points each for 4 different genotypes are included: wild-type (strain N2 grown on E. coli strain OP50), pie-1(zu154) (progeny of homozygous mutant mothers [Unc] of strain JJ532 grown on E. coli strain OP50), pie-1(zu154);pal-1(RNAi) (progeny of homozygous mutant mothers [Unc] of strain JJ532 grown on E. coli strain HT115 expressing pal-1 hairpin RNA), and mex-3(zu155);skn-1(RNAi) (progeny of homozygous mutant mothers [Dpy] of strain JJ518 grown on E. coli strain HT115 expressing skn-1 hairpin RNA). Embryos were manually staged by morphology at the 4-cell stage and allowed to develop in water for defined amounts of time at 22 degrees C. RNA was amplified as described (Baugh et al. Development, 2003; Baugh et al. Nucleic Acids Research, 2001). This series of samples comprises all replicate data reported by Baugh et al. (Development, 2005).

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Andrew Hill 

PROVIDER: E-GEOD-2180 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The homeodomain protein PAL-1 specifies a lineage-specific regulatory network in the C. elegans embryo.

Baugh L Ryan LR   Hill Andrew A AA   Claggett Julia M JM   Hill-Harfe Kate K   Wen Joanne C JC   Slonim Donna K DK   Brown Eugene L EL   Hunter Craig P CP  

Development (Cambridge, England) 20050316 8


Maternal and zygotic activities of the homeodomain protein PAL-1 specify the identity and maintain the development of the multipotent C blastomere lineage in the C. elegans embryo. To identify PAL-1 regulatory target genes, we used microarrays to compare transcript abundance in wild-type embryos with mutant embryos lacking a C blastomere and to mutant embryos with extra C blastomeres. pal-1-dependent C-lineage expression was verified for select candidate target genes by reporter gene analysis, t  ...[more]

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