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Mock or latently infected KSHV cells (BCBL, SLK and HFF)

ABSTRACT: Mock or latently infected KSHV cells (BCBL, SLK and HFF)

PROVIDER: PRJNA125033 | ENA |

REPOSITORIES: ENA

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Mock or latently infected KSHV cells (BCBL, SLK and HFF)

Project description:[original title] Mock or latently infected KSHV cells (BCBL, SLK and HFF) vs common reference (mixture of RNA from both infected and uninfected cells). Expression profiling of latently infected cells using a custom tiling microarray. SLK and HFF cells were infected and selected for rKSHV.219. Mock infected SLK and HFF cells served as controls for each of these two stably infected cells, respectively. BJAB cells served as uninfected controls for the BCBL-1 cells. Biological replicates were harvested and analyzed.

2010-03-24 | GSE20443 | GEO

Mock or latently infected KSHV cells (BCBL, SLK and HFF)

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Mock or KSHV cells (BJABJ, BCBL, TIME and HUVEC) vs common reference (mixture of RNA from both infected and uninfected cells)

Project description:Expression profiling of latently infected cells using a custom tiling microarray HUVEC and TIME cells were infected BCBL-1-derived KSHV. Mock infected HUVEC and TIME cells served as controls for each of these two stably infected cells, respectively. BJAB cells served as uninfected controls for the BCBL-1 cells. KSHV-infected cells are induced to enter lytic cycle with valproate or Adenovirus-RTA. Cells were harvested at indicated time points and analyzed. Three condition experiment: mock infected, latently infected cells and lytically infected. Three cell types (BJAB cells served as uninfected controls for the BCBL-1 cells).

2010-07-07 | E-GEOD-21972 | biostudies-arrayexpress

Ago HITS-CLIP in KSHV-infected primary effusion lymphoma (PEL) cell lines BCBL-1 and BC-3

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Mock or KSHV cells (BJABJ, BCBL, TIME and HUVEC) vs common reference (mixture of RNA from both infected and uninfected cells)

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Project description:Human SLK cells were infected with wildtype (wt) and LANA knockout (KO) Kaposi's sarcoma-associated herpesvirus (KSHV), separately for 3 days. Cellular gene expression changes were identified upon the wild type and LANA KO KSHV virus infection compared to the uninfected SLK cells using the human gene expression microarray U133plus2.0. 2 independent biological replicates from uninfected SLK cells, wild type KSHV infected SLK cells at 72hrs post-infection (hpi) , and LANA KO infected SLK cells at 72 hrs post-infection were collected and RNA was prepared for microarray analysis.

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Effect of LANA deletion on the host gene expression during de novo KSHV infection of SLK cells.

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ZIC2 ChIP-Seq in KSHV latent and lytic reactivated BCBL-1 cells

Project description:ZIC2 is required for the maintenance of KSHV latency in human host cells. To understand the molecular action of ZIC2, we map ZIC2 binding sites across the KSHV genome in BCBL-1 cells using ChIP-Seq analysis.

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Ectopic expression of viral circRNAs in SLK cell line

Project description:Total RNA was extracted from SLK cells that constitutively express select KSHV circular RNAs, and transcriptome analysis was performed by deep-sequencing (PE150).

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