Unknown,Transcriptomics,Genomics,Proteomics

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Mock or latently infected KSHV cells (BCBL, SLK and HFF)


ABSTRACT: [original title] Mock or latently infected KSHV cells (BCBL, SLK and HFF) vs common reference (mixture of RNA from both infected and uninfected cells). Expression profiling of latently infected cells using a custom tiling microarray. SLK and HFF cells were infected and selected for rKSHV.219. Mock infected SLK and HFF cells served as controls for each of these two stably infected cells, respectively. BJAB cells served as uninfected controls for the BCBL-1 cells. Biological replicates were harvested and analyzed. Two condition experiment: mock infected vs. latently infected cells. Three cell types.

ORGANISM(S): Homo sapiens

SUBMITTER: Sanjay Chandriani 

PROVIDER: E-GEOD-20443 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Array-based transcript profiling and limiting-dilution reverse transcription-PCR analysis identify additional latent genes in Kaposi's sarcoma-associated herpesvirus.

Chandriani Sanjay S   Ganem Don D  

Journal of virology 20100310 11


Kaposi's sarcoma-associated herpesvirus (KSHV) is a B-lymphotropic herpesvirus strongly linked to both lymphoproliferative diseases and Kaposi's sarcoma. The viral latency program of KSHV is central to persistent infection and plays important roles in the pathogenesis of KSHV-related tumors. Up to six polypeptides and 18 microRNAs are known to be expressed in latency, but it is unclear if all major latency genes have been identified. Here, we have employed array-based transcript profiling and li  ...[more]

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