Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of jejunum from iron-replete or iron-deficient rats

ABSTRACT: 12-week-old rats, iron-replete or iron-deficient. Three groups of each condition. 230A and 230B chips used with cRNA derived from jejunal scrapes.

ORGANISM(S): Rattus norvegicus

SUBMITTER: James Forrest Collins

PROVIDER: E-GEOD-2269 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Gene chip analyses reveal differential genetic responses to iron deficiency in rat duodenum and jejunum.

Collins James F JF

Biological research 20060101 1

Previous studies revealed novel genetic changes in the duodenal mucosa of iron-deprived rats during postnatal development. These observations are now extended to compare the genetic response to iron deficiency in the duodenum versus jejunum of 12-wk-old rats. cRNA samples were prepared from the duodenal and jejunal mucosa of three groups each of control and iron-deficient rats and hybridized with RAE 230A and 230B gene chips (Affymetrix). Stringent data reduction strategies were employed. Result ...[more]

PMID: 16629162

Similar Datasets

12 Week Rat Jejunum

Project description:12-week-old rats, iron-replete or iron-deficient. Three groups of each condition. 230A and 230B chips used with cRNA derived from jejunal scrapes. Keywords: ordered

2005-07-11 | GSE2269 | GEO

Transcription profiling of rat duodenum and jejunum from Belgrade +/b and b/b iron deficient rats

Project description:Three groups of male +b and bb rats were obtained (ages between 6 and 14 months) and intestinal scrapes were taken. Tissues was combined from 3 rats per group and processed for gene chip analysis. Experiment Overall Design: Three groups of each genotype were utilized and samples from each group of rats was reacted with one chip. 6 total experimental groups X 2 tissues (duodenum and jejunum) = 12 total chips used.

2008-04-06 | E-GEOD-7267 | biostudies-arrayexpress

Global gene expression in Moraxella catarrhalis ATCC 43617 grown under iron-replete and iron-limiting conditions

Project description:Total RNA samples were isolated from M. catarrhalis grown in BHI (iron-replete condition, designated DF0) and in BHI containing 30 mM Desferal (iron-limiting condition, designated DF30). Total RNA and M. catarrhalis genome-directed primers (GDPs) were used for synthesis of Cy3- or Cy5-labeled cDNA samples. Three independent M. catarrhalis growth experiments and total RNA isolations were used for synthesis of cDNA samples, and a total of six DNA microarray hybridizations were performed in this study. Keywords: Effect of Growth Environment on Global Gene Expression by Moraxella catarrhalis

2007-12-07 | GSE4346 | GEO

Xanthomonas campestris pv. campestris str. 8004 (Xcc 8004; Indian Isolate): Wild type vs xibR mutant, under the both iron-replete and iron-deplete conditions.

Project description:Transcriptional profiling of low-iron stimulon and XibR influenced regulon using wild-type Xcc 8004 and xibR mutant grown under iron-replete and iron-deplete conditions. Trancriptional analysis under iron-deplete condition, mimicking in planta environment, provides greater insights into expression pattern of several virulence-associated functions under low-iron. A genetic screen sggested the involvement of XibR (Xanthomonas iron binding regulator) in iron-uptake and metabolism. Present transcriptional analysis suggested the co-regulation of virulence associated functions including siderophore biosynthesis, motility, chemotaxis and typeIII effectors by a novel transcriptional regulator of NtrC family protein XibR and iron avability.

2016-11-02 | GSE89267 | GEO

Effect of maternal iron deficiency on placental gene expression on gestational day 18.5 in rats

Project description:Iron deficiency occurs when iron demands chronically exceed intake, and is particularly prevalent in pregnant women. Iron deficiency during pregnancy poses health risks for the baby. The placenta serves as the interface between a pregnant mother and her baby; thus, maternal iron deficiency may indirectly impact fetal growth and development by altering placental function. In this study, pregnant Sprague-Dawley rats were fed either a low-iron or iron-replete diet starting two weeks before mating. On gestational day 18.5, RNA was collected, and a Clariom S microarray was performed to elucidate differences in gene expression between gestaional day 18.5 placentas isolated from dams fed iron replete or iron deficient diets.

2021-11-10 | GSE163226 | GEO

Profiling of the Cellular Proteome and Phosphoproteome of Klebsiella pneumoniae under Iron Limited and Iron Replete Conditions

Project description:Klebsiella pneumoniae was compared across iron limited and iron replete conditions to assess changes within the cellular proteome and phosphoproteome using quantitative mass spectrometry. These comparative proteomic data provide insight into cellular response to nutrient limitation and how nutrient requirements could be exploited to provide potential antimicrobial targets.

2023-03-09 | PXD040658 | Pride

Wistar rats with iron deficiency and repletion and Belgrade rats normally fed or fed iron in drinking water: villus

Project description:Gene expression along the crypt-villus (C-V) axis was analyzed using cryostat sectioning to isolate fractions representing the crypts (bottom) and villus tops (top). These fractions were used for analyzing gene expression in iron replete Wistar rats (++), iron deficient Wistar rats (low iron), and in iron deficient Wistar rats fed iron for 3 and 6 days (iron-fed). Differences were observed between the crypts and villus tops in the expression of genes associated with Wnt and BNP signaling, cell proliferation and apoptosis, lipid and iron transport and metabolism. Gene expression in villus crypts and tops was also compared between Wistar and Belgrade rats (bb) and Belgrade rats fed iron (iron-fed) particularly as related to iron absorption and metabolism to define the affects of the mutation in DMT1 in the Belgrade rat on the expression of genes related to iron absorption and metabolism and the response to iron feeding. Keywords: iron stress response

2010-05-01 | GSE7970 | GEO

Belgrade +/b and b/b Duodenum and Jejunum

2008-03-03 | GSE7267 | GEO

Bordetella bronchiseptica RB50 iron limitation 2

Project description:Bordetella bronchiseptica RB50 was shifted from iron replete to either iron depleted or iron replete media, and samples were taken post shift for transcriptional profiling

2011-09-08 | E-MEXP-3076 | biostudies-arrayexpress

A Liver Hepcidin-Intestinal HIF-2α Axis Regulates Iron Absorption During Systemic Iron Deficiency, Hypoxia and Hemochromatosis

Project description:Iron-related disorders are among the most prevalent diseases worldwide. Systemic iron homeostasis requires hepcidin (Hamp), a hepatic-derived hormone that controls iron mobilization through its molecular target, ferroportin (FPN), the only known mammalian iron exporter. Here, we took a transcriptomic approach to to compare the duodenal transcriptome during systemic iron demand to that of hepcidin-deficiency iron overload. Hampfl/fl (control) and AlbCreERT2;Hampfl/fl mice were placed on iron-replete and low-iron diets and were sacrificed two weeks following tamoxifen treatment. Duodenum RNA expression was compared across genotypes and across iron-replete and low iron diets.

2018-12-12 | E-MTAB-7329 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data