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Transcription profiling of rat duodenum and jejunum from Belgrade +/b and b/b iron deficient rats

ABSTRACT: Three groups of male +b and bb rats were obtained (ages between 6 and 14 months) and intestinal scrapes were taken. Tissues was combined from 3 rats per group and processed for gene chip analysis. Experiment Overall Design: Three groups of each genotype were utilized and samples from each group of rats was reacted with one chip. 6 total experimental groups X 2 tissues (duodenum and jejunum) = 12 total chips used.

ORGANISM(S): Rattus norvegicus

SUBMITTER: James Collins 

PROVIDER: E-GEOD-7267 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Induction of arachidonate 12-lipoxygenase (Alox15) in intestine of iron-deficient rats correlates with the production of biologically active lipid mediators.

Collins James F JF   Hu Zihua Z   Ranganathan P N PN   Feng Dian D   Garrick Laura M LM   Garrick Michael D MD   Browne Richard W RW  

American journal of physiology. Gastrointestinal and liver physiology 20080207 4

To identify novel genes associated with iron metabolism, we performed gene chip studies in two models of iron deficiency: iron-deprived rats and rats deficient in the principal intestinal iron transporter, divalent metal transporter 1 (i.e., Belgrade rats). Affymetrix rat genome gene chips were utilized (RAE230) with cRNA samples derived from duodenum and jejunum of experimental and control animals. Computational analysis and statistical data reduction identified 29 candidate genes, which were i  ...[more]

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