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FTO: gene expression and function in skeletal muscle

ABSTRACT: We identified the target genes of FTO ("fat mass and obesity associated") in primary cultures of human skeletal muscle cells using adenoviral vectors expressing FTO or GFP and oligonucleotide microarrays. Human myotubes were prepared from 4 different skeletal muscle biopsies. After differentiation, myotubes were infected for 48 hours with recombinant adenovirus expressing either GFP or FTO. Each FTO-infected myotubes culture was compared to GFP-infected myotubes culture. GFP-infected myotubes were regarded as the control. Four biological replicates were processed.

ORGANISM(S): Homo sapiens

SUBMITTER: emmanuelle meugnier

PROVIDER: E-GEOD-22857 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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FTO is increased in muscle during type 2 diabetes, and its overexpression in myotubes alters insulin signaling, enhances lipogenesis and ROS production, and induces mitochondrial dysfunction.

Bravard Amélie A Lefai Etienne E Meugnier Emmanuelle E Pesenti Sandra S Disse Emmanuel E Vouillarmet Julien J Peretti Nöel N Rabasa-Lhoret Rémi R Laville Martine M Vidal Hubert H Rieusset Jennifer J

Diabetes 20101013 1

<h4>Objective</h4>A strong association between genetic variants and obesity was found for the fat mass and obesity-associated gene (FTO). However, few details are known concerning the expression and function of FTO in skeletal muscle of patients with metabolic diseases.<h4>Research design and methods</h4>We investigated basal FTO expression in skeletal muscle from obese nondiabetic subjects and type 1 and type 2 diabetic patients, compared with age-matched control subjects, and its regulation in ...[more]

PMID: 20943749

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