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Expression of 50-500nt ncRNAs in silkworm Bombyx mori during development

ABSTRACT: With an experimental RNomics, we identified 194 novel ncRNAs in silkworm through four developmental stages (egg, larva, pupa and adult). Oligos of 132 ncRNAs were sucessfully designed, the expression of these ncRNAs were analyzed by dual-channel microarray, results showed that 36 ncRNAs had significantly differential expression during development. The RNA from four samples (egg, larva, pupa and adult) were analyzed, the sample of larva was used as reference.

ORGANISM(S): Bombyx mori

SUBMITTER: Yunchao Kan

PROVIDER: E-GEOD-22911 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression of 50-500nt ncRNAs in silkworm Bombyx mori during two developmental transitions

Project description:We have performed the first systematic identification and analysis of intermediate size ncRNAs (50-500 nt) in the silkworm genome. We identified 194 novel ncRNAs, the expression profiles of them during the transitions from the egg to the first instar larva and from the fifth instar larva to the pupa were anlyzed by dual-channel microarray. Results showed that 12 ncRNAs had significantly differential expression during the two developmental transitions. The first instar larva sample was used for comparison to the sample of embryo, the fifth instar larva sample was used for comparison to the sample of pupa, respectively.

2010-12-22 | E-GEOD-22912 | biostudies-arrayexpress

Expression of 50-500nt ncRNAs in silkworm Bombyx mori during development

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Expression of 50-500nt ncRNAs in silkworm Bombyx mori during two developmental transitions

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Expression of 50-500nt ncRNAs in silkworm Bombyx mori: developmental time courses

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