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HapX positively and negatively regulates the transcriptional response to iron deprivation in Cryptococcus neoformans

ABSTRACT: Analysis of the transcriptional response of C. neoformans WT, cir1 mutant, hap3 mutant and hapX mutant to different iron sources. The following experimental design was adopted for the study. Within each strain, each treatment pair (low-iron, +FeCl3, +Transferrin and +Hemin; 6 pairs) were hybridized to an array; and within each treatment, each strain (WT, hap3, hapX and cir1, 6 pairs) were hybridized to an array for a total of 48 microarrays. Each strain/treatment combination was labeled an equal number of times with Cy3 and Cy5 to ensure dye balance

ORGANISM(S): Cryptococcus neoformans

SUBMITTER: Won Hee Jung

PROVIDER: E-GEOD-22988 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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HapX positively and negatively regulates the transcriptional response to iron deprivation in Cryptococcus neoformans.

Jung Won Hee WH Saikia Sanjay S Hu Guanggan G Wang Joyce J Fung Carlen Ka-Yin CK D'Souza Cletus C White Rick R Kronstad James W JW

PLoS pathogens 20101124 11

The fungal pathogen Cryptococcus neoformans is a major cause of illness in immunocompromised individuals such as AIDS patients. The ability of the fungus to acquire nutrients during proliferation in host tissue and the ability to elaborate a polysaccharide capsule are critical determinants of disease outcome. We previously showed that the GATA factor, Cir1, is a major regulator both of the iron uptake functions needed for growth in host tissue and the key virulence factors such as capsule, melan ...[more]

PMID: 21124817

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