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Expression data from BAP1 depleted cells

ABSTRACT: The deubiquitinase BAP1 is a candidate tumor suppressor regulating cell proliferation in human and is required for development in Drosophila. BAP1 is assembled into high molecular weight transcriptional multi-protein complexes. In order to identify potential BAP1 target genes, global mRNA expression profiling using microarrays was conducted. U2OS cells, transfected with a non-target control shRNA or shRNAs targeting BAP1, were selected with puromycin containing medium and then synchronized at the G1/S border to allow comparative analysis of gene expression.

ORGANISM(S): Homo sapiens

SUBMITTER: El Bachir Affar

PROVIDER: E-GEOD-23035 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The ubiquitin carboxyl hydrolase BAP1 forms a ternary complex with YY1 and HCF-1 and is a critical regulator of gene expression.

Yu Helen H Mashtalir Nazar N Daou Salima S Hammond-Martel Ian I Ross Julie J Sui Guangchao G Hart Gerald W GW Rauscher Frank J FJ Drobetsky Elliot E Milot Eric E Shi Yang Y Affar El Bachir el B

Molecular and cellular biology 20100830 21

The candidate tumor suppressor BAP1 is a deubiquitinating enzyme (DUB) involved in the regulation of cell proliferation, although the molecular mechanisms governing its function remain poorly defined. BAP1 was recently shown to interact with and deubiquitinate the transcriptional regulator host cell factor 1 (HCF-1). Here we show that BAP1 assembles multiprotein complexes containing numerous transcription factors and cofactors, including HCF-1 and the transcription factor Yin Yang 1 (YY1). Throu ...[more]

PMID: 20805357

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