Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profling of glioblastoma stem-like (GS) cell lines, conventional glioma cell lines and primary tumors

ABSTRACT: We compared a large panel of human glioblastoma stem-like (GS) cell lines, corresponding primary tumors and conventional glioma cell lines to identify cell lines that preserve the transcriptome of human glioblastomas most closely, thereby allowing identification of shared therapeutic targets. We used Affymetrix HG-U133 Plus 2.0 microarrays to compare human glioblastoma stem-like (GS) cell lines, corresponding primary tumors and conventional glioma cell lines. We extracted total RNA from 32 conventional glioma cell lines, 12 GS cell lines (8 in two different passages), 7 clonal sublines derived from two GS lines, 12 original tumors, and 4 monolayer cultures established from the same tumors as GS-lines using standard serum conditions.

ORGANISM(S): Homo sapiens

SUBMITTER: Hauke Günther 

PROVIDER: E-GEOD-23806 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A distinct subset of glioma cell lines with stem cell-like properties reflects the transcriptional phenotype of glioblastomas and overexpresses CXCR4 as therapeutic target.

Schulte Alexander A   Günther Hauke S HS   Phillips Heidi S HS   Kemming Dirk D   Martens Tobias T   Kharbanda Samir S   Soriano Robert H RH   Modrusan Zora Z   Zapf Svenja S   Westphal Manfred M   Lamszus Katrin K  

Glia 20110203 4

Glioblastomas contain stem-like cells that can be maintained in vitro using specific serum-free conditions. We investigated whether glioblastoma stem-like (GS) cell lines preserve the expression phenotype of human glioblastomas more closely than conventional glioma cell lines. Expression profiling revealed that a distinct subset of GS lines, which displayed a full stem-like phenotype (GSf), mirrored the expression signature of glioblastomas more closely than either other GS lines or cell lines g  ...[more]

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