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Gene expression analysis for Il13Ra2-positive and IL13Ra2-negative glioma cell lines

ABSTRACT: Affymetrix expression profiling was used to evaluate the association between IL13Rα2 expression, and mesenchymal, proneural, classical and neural signature genes expression for glioma subclasses defined by Verhaak et al (Cancer Cell; 2010). The goal of this study is to compare gene expression patterns between IL13Ra2-postive and IL13Ra2-negative glioma cell lines. IL13Ra2 status was determined by flow cytometry and confirmed by qPCR.

ORGANISM(S): Homo sapiens

SUBMITTER: Charles Warden

PROVIDER: E-GEOD-40904 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Glioma IL13Rα2 is associated with mesenchymal signature gene expression and poor patient prognosis.

Brown Christine E CE Warden Charles D CD Starr Renate R Deng Xutao X Badie Behnam B Yuan Yate-Ching YC Forman Stephen J SJ Barish Michael E ME

PloS one 20131018 10

A major challenge for successful immunotherapy against glioma is the identification and characterization of validated targets. We have taken a bioinformatics approach towards understanding the biological context of IL-13 receptor α2 (IL13Rα2) expression in brain tumors, and its functional significance for patient survival. Querying multiple gene expression databases, we show that IL13Rα2 expression increases with glioma malignancy grade, and expression for high-grade tumors is bimodal, with appr ...[more]

PMID: 24204956

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Gene expression analysis for Il13Ra2-positive and IL13Ra2-negative glioma cell lines

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2014-01-29 | GSE40904 | GEO

Transcription profling of glioblastoma stem-like (GS) cell lines, conventional glioma cell lines and primary tumors

Project description:We compared a large panel of human glioblastoma stem-like (GS) cell lines, corresponding primary tumors and conventional glioma cell lines to identify cell lines that preserve the transcriptome of human glioblastomas most closely, thereby allowing identification of shared therapeutic targets. We used Affymetrix HG-U133 Plus 2.0 microarrays to compare human glioblastoma stem-like (GS) cell lines, corresponding primary tumors and conventional glioma cell lines. We extracted total RNA from 32 conventional glioma cell lines, 12 GS cell lines (8 in two different passages), 7 clonal sublines derived from two GS lines, 12 original tumors, and 4 monolayer cultures established from the same tumors as GS-lines using standard serum conditions.

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Carboxypeptidase E transmits its anti-migratory function in glioma cells via transcriptional regulation of cell architecture and motility regulating factors.

Project description:They aim of the study is to identify targets of Carboxypeptidase E (CPE) as well as signaling cascades that are affected by CPE which are specific for transmitting its anti-migratory effects in glioma cells by overexpressing CPE and using transcriptomics profiling of mRNAs and microRNAs.

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