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Chromatin function of IkBa: ChIP-on-chip of IkBa

ABSTRACT: The Inhibitor of the NFkB, IkBa, is the main regulator of the NFkB pathway. Our data has demonstrated that there is an alternative function for IkBa in the chromatin that affects HOX and other developmental genes. Keywords: CHIP-chip Chip-on-chip analysis has demonstrated that IkBa associates to the promoters of several genes. Chromatin fragments were obtained from NIH-3T3 cells and precipitated with 3 different IkBa comercial antibodies (raised in mouse, goat and rabbit), and fold enrichment was compared to input.

ORGANISM(S): Mus musculus

SUBMITTER: Lauro Sumoy

PROVIDER: E-GEOD-24011 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Chromatin-bound IκBα regulates a subset of polycomb target genes in differentiation and cancer.

Mulero María Carmen MC Ferres-Marco Dolors D Islam Abul A Margalef Pol P Pecoraro Matteo M Toll Agustí A Drechsel Nils N Charneco Cristina C Davis Shelly S Bellora Nicolás N Gallardo Fernando F López-Arribillaga Erika E Asensio-Juan Elena E Rodilla Verónica V González Jessica J Iglesias Mar M Shih Vincent V Mar Albà M M Di Croce Luciano L Hoffmann Alexander A Miyamoto Shigeki S Villà-Freixa Jordi J López-Bigas Nuria N Keyes William M WM Domínguez María M Bigas Anna A Espinosa Lluís L

Cancer cell 20130711 2

IκB proteins are the primary inhibitors of NF-κB. Here, we demonstrate that sumoylated and phosphorylated IκBα accumulates in the nucleus of keratinocytes and interacts with histones H2A and H4 at the regulatory region of HOX and IRX genes. Chromatin-bound IκBα modulates Polycomb recruitment and imparts their competence to be activated by TNFα. Mutations in the Drosophila IκBα gene cactus enhance the homeotic phenotype of Polycomb mutants, which is not counteracted by mutations in dorsal/NF-κB. ...[more]

PMID: 23850221

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