Unknown,Transcriptomics,Genomics,Proteomics

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50 uM AFB_time course


ABSTRACT: RNA isolated from the cultures treated with 0 and 50 micromolar AFB1 at 15, 30, 60, 90, 120 min was used for microarray experiments. For each array hybridization experiment, RNAs from the treated sample and its corresponding time-matched control were co-hybridized to arrays and respectively quantified in different channels. A dye swap strategy was used to eliminate the dye bias. Keywords: time-course

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Yingying Guo 

PROVIDER: E-GEOD-2420 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Analysis of cellular responses to aflatoxin B(1) in yeast expressing human cytochrome P450 1A2 using cDNA microarrays.

Guo Yingying Y   Breeden Linda L LL   Fan Wenhong W   Zhao Lue Ping LP   Eaton David L DL   Zarbl Helmut H  

Mutation research 20050824 1-2


Aflatoxin B1 (AFB(1)) is a potent human hepatotoxin and hepatocarcinogen produced by the mold Aspergillus flavus. In human, AFB(1) is bioactivated by cytochrome P450 (CYP450) enzymes, primarily CYP1A2, to the genotoxic epoxide that forms N(7)-guanine DNA adducts. To characterize the transcriptional responses to genotoxic insults from AFB(1), a strain of Saccharomyces cerevisiae engineered to express human CYP1A2 was exposed to doses of AFB(1) that resulted in minimal lethality, but substantial g  ...[more]

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