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Genome-wide analysis of the effect of cryopreservation on peripheral blood mononuclear cells

ABSTRACT: Analysis of cryopreservation effects on peripheral blood mononuclear cells at gene expression level. The hypothesis tested in the present study was that cryopreservation has an influence on the transcriptome profile of peripheral blood mononuclear cells. Results indicated remarkable changes in expression patterns upon cryopreservation of PBMCs, with a strong loss of signal intensities to background levels for several transcripts. Total RNA obtained from isolated peripheral blood mononuclear cells of healthy human subjects eihter cryopreserved in liqiud nitrogen (frozen) or direclty lysed in Trizol after isolation (fresh).

ORGANISM(S): Homo sapiens

SUBMITTER: Svenja Debey-Pascher 

PROVIDER: E-GEOD-24753 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RNA-stabilized whole blood samples but not peripheral blood mononuclear cells can be stored for prolonged time periods prior to transcriptome analysis.

Debey-Pascher Svenja S   Hofmann Andrea A   Kreusch Fatima F   Schuler Gerold G   Schuler-Thurner Beatrice B   Schultze Joachim L JL   Staratschek-Jox Andrea A  

The Journal of molecular diagnostics : JMD 20110701 4

Microarray-based transcriptome analysis of peripheral blood as surrogate tissue has become an important approach in clinical implementations. However, application of gene expression profiling in routine clinical settings requires careful consideration of the influence of sample handling and RNA isolation methods on gene expression profile outcome. We evaluated the effect of different sample preservation strategies (eg, cryopreservation of peripheral blood mononuclear cells or freezing of PAXgene  ...[more]

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