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Cryopreservation effects on peripheral blood

ABSTRACT: This SuperSeries is composed of the following subset Series: GSE24753: Genome-wide analysis of the effect of cryopreservation on peripheral blood mononuclear cells GSE24755: Genome-wide analysis of the effect of long-term cryopreservation on peripheral blood mononuclear cells GSE24757: Genome-wide analysis of the effect of long-term freezing of PAXgene Blood RNA tubes Refer to individual Series

ORGANISM(S): Homo sapiens

SUBMITTER: Svenja Debey-Pascher

PROVIDER: E-GEOD-24758 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RNA-stabilized whole blood samples but not peripheral blood mononuclear cells can be stored for prolonged time periods prior to transcriptome analysis.

Debey-Pascher Svenja S Hofmann Andrea A Kreusch Fatima F Schuler Gerold G Schuler-Thurner Beatrice B Schultze Joachim L JL Staratschek-Jox Andrea A

The Journal of molecular diagnostics : JMD 20110701 4

Microarray-based transcriptome analysis of peripheral blood as surrogate tissue has become an important approach in clinical implementations. However, application of gene expression profiling in routine clinical settings requires careful consideration of the influence of sample handling and RNA isolation methods on gene expression profile outcome. We evaluated the effect of different sample preservation strategies (eg, cryopreservation of peripheral blood mononuclear cells or freezing of PAXgene ...[more]

PMID: 21704280

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Genome-wide analysis of the effect of long-term cryopreservation on peripheral blood mononuclear cells

Project description:Analysis of effect of long-term cryopreservation on peripheral blood mononuclear cells at gene expression level. The hypothesis tested in the present study was that long-term cryopreservation has an influence on the transcriptome profile of peripheral blood mononuclear cells. Results indicated remarkable changes in expression patterns upon cryopreservation of PBMCs, with decreasing signal intensities over time. Total RNA obtained from isolated peripheral blood mononuclear cells of melanoma patients cryopreserved in liqiud nitrogen from 20 to 60 months.

2011-07-21 | E-GEOD-24755 | biostudies-arrayexpress

Genome-wide analysis of the effect of cryopreservation on peripheral blood mononuclear cells

Project description:Analysis of cryopreservation effects on peripheral blood mononuclear cells at gene expression level. The hypothesis tested in the present study was that cryopreservation has an influence on the transcriptome profile of peripheral blood mononuclear cells. Results indicated remarkable changes in expression patterns upon cryopreservation of PBMCs, with a strong loss of signal intensities to background levels for several transcripts. Total RNA obtained from isolated peripheral blood mononuclear cells of healthy human subjects eihter cryopreserved in liqiud nitrogen (frozen) or direclty lysed in Trizol after isolation (fresh).

2011-07-21 | E-GEOD-24753 | biostudies-arrayexpress

Genome-wide analysis of the effect of long-term cryopreservation on peripheral blood mononuclear cells

2011-07-22 | GSE24755 | GEO

Genome-wide analysis of the effect of cryopreservation on peripheral blood mononuclear cells

2011-07-22 | GSE24753 | GEO

Effect of Cryopreservation on Autologous Chimeric Antigen Receptor T Cell Characteristics

Project description:As clinical applications for chimeric antigen receptor T cell (CART) therapy extend beyond early phase trials, commercial manufacture incorporating preservation steps becomes a logistical necessity. The effect of cryopreservation on CART characteristics is unclear. We retrospectively evaluated the effect of cryopreservation on product release criteria and in vivo characteristics in 158 autologous CART products from 6 single-center clinical trials. Further, from 3 healthy donor manufacturing runs, we prospectively identified differentially expressed cell surface markers and gene signatures among fresh versus cryopreserved CARTs. Within 2 days of culture initiation, cell viability of the starting fraction (peripheral blood mononuclear cells [PBMNCs]) decreased significantly in the cryo-thawed arm compared to the fresh arm. Despite this, PBMNC cryopreservation did not affect final CART fold expansion, transduction efficiency, CD3%, or CD4:CD8 ratios. In vivo CART persistence and clinical responses did not differ among fresh and cryopreserved final products. In healthy donors, compared to fresh CARTs, early apoptotic cell-surface markers were significantly elevated in cryo-thawed CARTs. Cryo-thawed CARTs also demonstrated significantly elevated expression of mitochondrial dysfunction, apoptosis signaling, and cell cycle damage pathways. Cryopreservation during CART manufacture is a viable strategy, based on standard product release parameters. The clinical impact of cryopreservationrelated subtle micro-cellular damage needs further study

2020-10-03 | GSE158929 | GEO

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The SENSE (Simple prEservatioN of Single cElls) method for cryopreservation enables the generation of single-cell immune profiles from whole blood.

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Genome-wide analysis of the effect of long-term freezing of PAXgene Blood RNA tubes

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