Transcriptomics

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The SENSE (Simple prEservatioN of Single cElls) method for cryopreservation enables the generation of single-cell immune profiles from whole blood.


ABSTRACT: We developed and validated a simple method for viable cryopreservation of whole blood, without any preprocessing, Simple prEservatioN of Single cElls (SENSE), with granulocyte depletion for generating high-quality single-cell profiles. We performed rigorous in-depth characterization of the SENSE method on clinical blood samples and compared it to the conventional multistep density-gradient isolation of peripheral blood mononuclear cells (PBMCs) method. The SENSE method is an effective and simple solution for the cryopreservation of blood samples in clinics/labs and single cell profiles generation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE226557 | GEO | 2023/11/28

REPOSITORIES: GEO

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