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Neonatal beta cells lack the specialized metabolic phenotype of mature beta cells


ABSTRACT: Fetal and neonatal beta cells have poor glucose-induced insulin secretion and only gain robust glucose responsiveness several weeks after birth. This unresponsiveness may be due to a generalized immaturity of the metabolic pathways normally found in beta cells. Gene expression profile of neonatal (1 day old) and young adult (6 weeks old) Sprague-Dawley rat islets were evaluated and compared. Frozen sections were obtained from pancreases of neonatal (1 day old) and young adult (6 weeks old) Sprague-Dawley rats. The beta cell enriched cores of the islets were excised using laser capture microdissection. RNA was extracted, amplified and subjected to microarray analysis.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Susan Bonner-Weir PhD 

PROVIDER: E-GEOD-24790 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Rat neonatal beta cells lack the specialised metabolic phenotype of mature beta cells.

Jermendy A A   Toschi E E   Aye T T   Koh A A   Aguayo-Mazzucato C C   Sharma A A   Weir G C GC   Sgroi D D   Bonner-Weir S S  

Diabetologia 20110116 3


<h4>Aims/hypothesis</h4>Fetal and neonatal beta cells have poor glucose-induced insulin secretion and only gain robust glucose responsiveness several weeks after birth. We hypothesise that this unresponsiveness is due to a generalised immaturity of the metabolic pathways normally found in beta cells rather than to a specific defect.<h4>Methods</h4>Using laser-capture microdissection we excised beta cell-enriched cores of pancreatic islets from day 1 (P1) neonatal and young adult Sprague-Dawley r  ...[more]

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