Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression profiling of Schistosoma mansoni larval development using serial analysis of gene expression (SAGE)

ABSTRACT: Biomphalaria glabrata infection by the Schistosoma mansoni free-swimming miracidium and its subsequent development to the parasitic sporocyst stage is critical to establishment of viable infections and triggers a variety of physiological, biochemical and molecular changes. Here, we describe a genome-wide analysis of the S. mansoni miracidium and developing sporocyst. Keywords: life-cycle, development, host-interaction We generated transcriptomic profiles of the developing larval stages of Schistosoma mansoni using long serial analysis of gene expression (LongSAGE). Five cDNA libraries were constructed from miracidia and in vitro cultured 6- and 20-day old sporocysts maintained in sporocyst medium (SM) or in SM conditioned by previous cultivation with cells of the B. glabrata embryonic (Bge) cell line. From five libraries, 314,799 SAGE tags were sequenced and resulted in a total of 21,440 unique sequence tags. A total of 254 tags were differentially expressed during “conditioned” development and 236 tags were differentially expressed during “un-conditioned” development. In addition, 53 tags were found to be differentially expressed between 6-day conditioned and unconditioned sporocysts and 42 tags between 20-day conditioned and unconditioned sporocysts.

ORGANISM(S): Schistosoma mansoni

SUBMITTER: Andrew McArthur

PROVIDER: E-GEOD-9722 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome analysis of Schistosoma mansoni larval development using serial analysis of gene expression (SAGE).

Taft A S AS Vermeire J J JJ Bernier J J Birkeland S R SR Cipriano M J MJ Papa A R AR McArthur A G AG Yoshino T P TP

Parasitology 20090305 5

<h4>Summary</h4>Infection of the snail, Biomphalaria glabrata, by the free-swimming miracidial stage of the human blood fluke, Schistosoma mansoni, and its subsequent development to the parasitic sporocyst stage is critical to establishment of viable infections and continued human transmission. We performed a genome-wide expression analysis of the S. mansoni miracidia and developing sporocyst using Long Serial Analysis of Gene Expression (LongSAGE). Five cDNA libraries were constructed from mira ...[more]

PMID: 19265565

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Dataset Information

Gene expression profiling of Schistosoma mansoni larval development using serial analysis of gene expression (SAGE)

Publications

Transcriptome analysis of Schistosoma mansoni larval development using serial analysis of gene expression (SAGE).

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