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Genome-wide analysis gene expression in SUM-149 cells expressing AREG shRNA

ABSTRACT: Results of knocking-down AREG expression in SUM-149 cells by lenitviral infection of shRNA vectors and measuring gene expression provides information as to what genes are regulated by AERG in inflammatory breast cancer cells. SUM-149 cells were infected with a single AREG shRNA virus (sh4) or three AREG shRNA viruses (shPld) and the non-silencing vector (shNS). Total RNA was collected and genome-wide analysis of expression was performed on RNA from each cell line.

ORGANISM(S): Homo sapiens

SUBMITTER: Andrea Baillo

PROVIDER: E-GEOD-26079 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide analysis gene expression in SUM-149 cells expressing AREG shRNA

Project description:Results of knocking-down AREG expression in SUM-149 cells by lenitviral infection of shRNA vectors and measuring gene expression provides information as to what genes are regulated by AERG in inflammatory breast cancer cells.

2010-12-15 | GSE26079 | GEO

Homo sapiens

Project description:Genome-wide analysis gene expression in SUM-149 cells expressing AREG shRNA

| PRJNA135213 | ENA

Genome-wide analysis of time-dependent gene expression in SUM-225 cells treated with the HER-2-specific inhibitor CP724,714 for 45 hours

Project description:Results of blocking the HER-2 oncogene kinase function in SUM-225 cells by treatment with CP724,714 and measuring gene expression as a function of time provides information as to what genes are regulated by HER-2 in this breast cancer cell line. SUM-225 cells were treated with the HER-2-specific small molecule kinase inhibitor CP724,714 for 45 hours. Total RNA was collected every 3 hours from parallel cultures throughout the 45 hour duration of treatment and genome-wide analysis of expression was performed on RNA from each time point (total 16 time points, starting at 0 hours treatment).

2011-03-18 | E-GEOD-22955 | biostudies-arrayexpress

Influence of NSD3 and ESR1 knockdown on gene expression in SUM-44 cells

Project description:Total RNA was isolated from SUM-44 cells subjected to 5 days of puromycin selection following infection with lentiviral shRNA constructs against NSD3-S or ESR1 as well as LacZ control. Samples were harvested in triplicate and prepared and sequenced simultaneously.

2018-09-01 | E-MTAB-5823 | biostudies-arrayexpress

Genome-wide analysis gene expression of MCF10A cells growing in the presence of EGF or AREG or without EGF or AREG for 24 hrs

Project description:Results of growing MCF10A cells continuously in serum free media supplemented with EGF (MCF10A) or AREG (MCF10A+AREG) followed by 24 hours of ligand withdrawl and measuring gene expression provides information as to what genes are regulated by AREG and EGF in a normal mammary epithelial cell model MCF10A cells continuously in serum free media supplemented with 10ng/ml of EGF (MCF10A) or 20ng/ml of AREG (MCF10A+AREG) followed by 24 hours of ligand withdrawl. Total RNA was collected and genome-wide analysis of expression was performed on RNA from each cell line.

2010-12-23 | E-GEOD-26264 | biostudies-arrayexpress

Genome-wide analysis of gene expression perturbed by Pontin shRNA in MCF7 subjected to normoxic and hypoxic conditions

2011-12-02 | GSE27813 | GEO

Gene Expression data from SH3GL2 overexpressing SUM-159 cells

Project description:Gene Expression data from SH3GL2 overexpressing SUM-159 cells

| PRJNA433484 | ENA

Transcriptomic profiling of CXCR3+/- SUM-159-LM1 breast cancer cells

Project description:Transcriptomic profiling of CXCR3+/- SUM-159-LM1 breast cancer cells

| PRJNA499116 | ENA

Mouse_embryoid Body_Retinoic acid treatment

Project description:S6K1 Knockout mice has a lean phenotype and resitant to a High Fat Diet-induced obesity (Um S.H., Nature, 2004). Adipocyte differentiation consists of two step, first, commitment from stem cell to adipocyte progenitors and second, terminal differentiation from adipocyte progenitors to mature adipocyte. We studied S6K1-dependent gene expression regulation in early stage of commitment by employing ES cell(ESC)-Embryoid Bodies(EBs) differentiation model. We established stable ES cell lines infected with either control non-silencing shRNA (shNS) or S6K1 targetting shRNA (shS6K1). Each sample was treated with retinoic acid for 3 days to induce adipogenesis, then gene expression profile was analyzed employing microarrays and up-regulated and down-regulated genes were selected for analysis. Mouse Embryoid Bodies (approximately 1000 EBs per sample) were treated with retinoic acid (1ÂµM) for 3 days then collected for RNA extraction and hybridization on Affimetrix microarray. Two biological replicates were each performed for EBs prepared from either shNS ESCs or shS6K1 ESCs.

2010-05-14 | E-GEOD-20349 | biostudies-arrayexpress

Genome-wide analysis of gene expression perturbed by Pontin shRNA in MCF7 subjected to normoxic and hypoxic conditions

Project description:In order to investigate the role of Pontin methylation on the expression of hypoxia responsive genes across the whole genome, we performed a microarray analysis from RNAs isolated from MCF7 cells expressing either control shRNA (shNS) or Pontin shRNA(shPontin) in normoxic and hypoxic conditions. MCF7 cells were transiently transfected with either non-specific shRNA or Pontin shRNA in replicates. Cells were grown for 24 hours before being exposed to either normoxic (21% Oxygen) or hypoxic condition (1% oxygen) for 6,9 hours. Cells were then lysed and RNA isolated.

2011-12-02 | E-GEOD-27813 | biostudies-arrayexpress

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