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Epigenetic changes in B lymphocytes associated with house dust mite allergic asthma.


ABSTRACT: The increase in atopic diseases has occurred in such a short period of time that it becomes difficult to be attributed only to genetic factors, which usually need more prolonged time periods to manifest. In this setting during the last decade, the science of epigenetics has increasingly developed offering new perspectives and opening a new challenging research area. In this study we aimed to study the epigenetic patterns in B CD19+ Lymphocytes from healthy and allergic patients using the improved version of HELP assay. Our study focused specifically on DNA methylation in B CD19+ Lymphocytes isolated from whole blood of dust allergic patients (ALLERGY, n=3), aspirin intolerants (ASPIRIN INTOLERANT, n=3) and healthy subjects (CONTROL, n=3). We utilized a two-stage design involving genome-wide discovery followed by quantitative, single-locus validation. Each microarray consists of a two-color comparison of a methylation-sensitive representation of the genome (HpaII) with an internal methylation-insensitive control/reference (MspI).

ORGANISM(S): Homo sapiens

SUBMITTER: Marien Pascual 

PROVIDER: E-GEOD-26080 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Epigenetic changes in B lymphocytes associated with house dust mite allergic asthma.

Pascual Marien M   Suzuki Masako M   Isidoro-Garcia Maria M   Padrón Juana J   Turner Terrence T   Lorente Felix F   Dávila Ignacio I   Greally John M JM  

Epigenetics 20110901 9


Although there is no doubt about the influence of the genetic background in the onset of the allergic diseases, Epigenome-Wide Association Studies are needed to elucidate the possible relationship between allergic diseases and epigenomic dysregulation. In this study we aimed to analyze the epigenetic patterns, in terms of DNA methylation, of three well-characterized populations of house dust mite allergic subjects, aspirin-intolerant asthmatics and controls. As a first, genome-wide phase, we use  ...[more]

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