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Modulation of mRNA in human T-cell development (miRNA)

ABSTRACT: miRNA expression of Double Positive, Single Positive CD4+ and CD8+, and unsorted human thymocytes We used microarrays to identify miRNAs involved in T cell maturation

ORGANISM(S): Homo sapiens

SUBMITTER: Alberto Corradin

PROVIDER: E-GEOD-26157 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Modulation of microRNA expression in human T-cell development: targeting of NOTCH3 by miR-150.

Ghisi Margherita M Corradin Alberto A Basso Katia K Frasson Chiara C Serafin Valentina V Mukherjee Subhamoy S Mussolin Lara L Ruggero Katia K Bonanno Laura L Guffanti Alessandro A De Bellis Gianluca G Gerosa Gino G Stellin Giovanni G D'Agostino Donna M DM Basso Giuseppe G Bronte Vincenzo V Indraccolo Stefano S Amadori Alberto A Zanovello Paola P

Blood 20110506 26

Ontogenesis of T cells in the thymus is a complex process whose molecular control is poorly understood. The present study investigated microRNAs involved in human thymocyte differentiation by comparing the microRNA expression profiles of thymocytes at the double-positive, single-positive CD4(+) and single-positive CD8(+) maturation stages. Microarray analysis showed that each thymocyte population displays a distinct microRNA expression profile that reflects their developmental relationships. Mor ...[more]

PMID: 21551231

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Modulation of mRNA in human T-cell development

Project description:This SuperSeries is composed of the following subset Series: GSE26156: Modulation of mRNA in human T-cell development (expression) GSE26157: Modulation of mRNA in human T-cell development (miRNA) Refer to individual Series

2011-10-26 | E-GEOD-26158 | biostudies-arrayexpress

Modulation of mRNA in human T-cell development (expression)

Project description:Gene expression of Double Positive, and Single Positive CD4+ human thymocytes We used microarrays to identify genes involved in T cell maturation

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Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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Modulation of mRNA in human T-cell development (miRNA)

Project description:miRNA expression of Double Positive, Single Positive CD4+ and CD8+, and unsorted human thymocytes

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Identification of a circulating miRNA that is involved in meat yield and connects exosomes and longissimus dorsi muscle in Korean cattle steers

Project description:MicroRNAs (miRNAs) regulate gene expression. The aim of this study was to identify circulating miRNAs that are involved with meat yield and connect exosomes and longissimus dorsi (LD) muscle in Korean cattle steer. Thus, we performed analyses of the carcass characteristics, exosome extraction, bovine miRNA array, mature miRNA qPCR, bioinformatics, and qPCR. Our analysis of the carcass characteristics relative to the yield grade (YG) showed that the yield index (YI) and rib eye area were the highest, whereas the backfat thickness was the lowest for YG A cattle among the three YGs. We performed a miRNA microarray and a Venn diagram analysis to sort the circulating miRNAs that connect exosomes and LD muscle. Mature miRNA qPCR showed that miR-15a (r = 0.84), miR-26b (r = 0.91), and miR-29c (r = 0.92) had positive relationships with exosomes and LD muscle. In YG A cattle, miR-26b was considered to be a circulating miRNA connecting exosomes and LD muscle because the expression pattern of miR-26b was similar in the microarray and miRNA qPCR for miR-26b-targeted genes, where the expression levels of DIAPH3 and YOD1 were lower than those in YG C cattle. Our results suggest that circulating miR-26b may participate in cell maintenance by regulating DIAPH3 and YOD1 in the LD muscle of Korean cattle. 29 or 30 month Korean cattle steers were classified by yield grade(YG) A, B, and C. miRNA expression patterns for exosome and LD muscle were compared among YGs.

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2010-12-06 | E-GEOD-25691 | biostudies-arrayexpress

Breast Cancer Cell Secretome Analysis to Decipher miRNA Tumor Biology and Discover Potential Biomarkers

Project description:MicroRNA (miRNA/miR) miR526b and miR655 overexpressed tumor cell-free secretions promote breast cancer phenotypes in the tumor microenvironment (TME). However, the mechanisms of miRNA regulating TME have never been investigated. With mass spectrometry analysis of MCF7-miRNA-overexpressed versus miRNA-low MCF7-Mock tumor cell secretomes, we identified 34 novel secretory proteins coded by eight genes YWHAB, TXNDC12, MYL6B, SFN, FN1, PSMB6, PRDX4, and PEA15 those are differentially regulated. We used bioinformatic tools and systems biology approaches to identify these markers’ role in breast cancer. Gene ontology analysis showed that the top functions are related to apoptosis, oxidative stress, membrane transport, and motility, supporting miRNA-induced phenotypes. These secretory markers expression is high in breast tumors, and a strong positive correlation exists between upregulated markers’ mRNA expressions with miRNA cluster expression in luminal A breast tumors. Gene expression of secretome markers is higher in tumor tissues compared to normal samples, and immunohistochemistry data supported gene expression data. Moreover, both up and downregulated marker expressions are associated with breast cancer patient survival. miRNA regulates these marker protein expressions by targeting transcription factors of these genes. Premature miRNA (pri-miR526b and pri-miR655) are established breast cancer blood biomarkers. Here we report novel secretory markers upregulated by miR526b and miR655 (YWHAB, MYL6B, PSMB6, and PEA15) are significantly upregulated in breast cancer patients’ plasma, and are potential breast cancer biomarkers.

2023-05-10 | PXD031771 | Pride

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