Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Effect of biotin deficiency on gene expression in Aravbidopsis leaves


ABSTRACT: In addition to its essential metabolic functions biotin is suggested a critical role in regulating gene expression. The first committed enzyme in biotin biosynthesis in Arabidopsis, 7-keto-8-aminopelargonic acid synthase is encoded by At5g04620 (BIO4). We isolated a novel T-DNA insertion mutant of BIO4 (bio4-1) showing a spontaneous cell death phenotype that could be rescued both by exogenous biotin and genetic complementation. The bio4-1 plants exhibited massive accumulation of hydrogen peroxide. The T-DNA insertion in the bio4-1 mutant contains a Methionine Sulfoxide Reductase B9 (AT4G21850) under a constitutive 35S CaMV promoter. To dissect the specific effects of biotin defiency on gene expression on a genome wide scale, we compared expression of wt Col-0 leaves with bio4-1 mutants and bio4-1 mutants complemented with D-biotin. Expression in leaves of Col-0 wt, bio4-1 mutants with a T-DNA insertion in the 5`-UTR of BIO4, and bio4-1 mutants complemented with watering with 200µM D-biotin.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Günter Brader 

PROVIDER: E-GEOD-26631 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Biotin deficiency causes spontaneous cell death and activation of defense signaling.

Li Jing J   Brader Günter G   Helenius Elina E   Kariola Tarja T   Palva E Tapio ET  

The Plant journal : for cell and molecular biology 20120116 2


In addition to its essential metabolic functions, biotin has been suggested to play a critical role in regulating gene expression. The first committed enzyme in biotin biosynthesis in Arabidopsis, 7-keto-8-aminopelargonic acid synthase, is encoded by At5g04620 (BIO4). We isolated a T-DNA insertion mutant of BIO4 (bio4-1) with a spontaneous cell death phenotype, which was rescued both by exogenous biotin and genetic complementation. The bio4-1 plants exhibited massive accumulation of hydrogen per  ...[more]

Similar Datasets

2011-12-22 | GSE26631 | GEO
2015-09-23 | E-GEOD-73351 | biostudies-arrayexpress
| PRJNA883671 | ENA
2020-12-31 | E-MTAB-8969 | biostudies-arrayexpress
2019-10-15 | E-MTAB-7544 | biostudies-arrayexpress
2021-01-28 | PXD019603 | Pride
2015-07-30 | E-GEOD-66419 | biostudies-arrayexpress
2008-06-15 | E-GEOD-7211 | biostudies-arrayexpress
2024-12-09 | PXD052789 | Pride
2011-03-01 | E-GEOD-23940 | biostudies-arrayexpress