Unknown,Transcriptomics,Genomics,Proteomics

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Chromosome wide analysis of parental allele specific chromatin and DNA methylation

ABSTRACT: Imprinted genes are monoallelically expressed according to parental inheritance. The maternally and paternally expressed alleles are distinguished epigenetically by DNA methylation and histone modifications. RNA was extracted from MatDup.dist7 and PatDup.dist7 MEFs for hybridization to Affymetrix microarrays Comparsion of gene expression in MatDup.dist7 and PatDup.dist7 MEFs

ORGANISM(S): Mus musculus

SUBMITTER: Xiwei Wu

PROVIDER: E-GEOD-26720 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Chromosome-wide analysis of parental allele-specific chromatin and DNA methylation.

Singh Purnima P Wu Xiwei X Lee Dong-Hoon DH Li Arthur X AX Rauch Tibor A TA Pfeifer Gerd P GP Mann Jeffrey R JR Szabó Piroska E PE

Molecular and cellular biology 20110214 8

To reveal the extent of domain-wide epigenetic features at imprinted gene clusters, we performed a high-resolution allele-specific chromatin analysis of over 100 megabases along the maternally or paternally duplicated distal chromosome 7 (Chr7) and Chr15 in mouse embryo fibroblasts (MEFs). We found that reciprocal allele-specific features are limited to imprinted genes and their differentially methylated regions (DMRs), whereas broad local enrichment of H3K27me3 (BLOC) is a domain-wide feature a ...[more]

PMID: 21321082

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Dataset Information

Chromosome wide analysis of parental allele specific chromatin and DNA methylation

Publications

Chromosome-wide analysis of parental allele-specific chromatin and DNA methylation.

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