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Chromosome wide analysis of parental allele specific chromatin and DNA methylation along mouse distal chr7

ABSTRACT: Imprinted genes are monoallelically expressed according to parental inheritance. The maternally and paternally inherited alleles are distinguished epigenetically by DNA methylation and histone modifications. Chromosome-wide Chromatin immunoprecipitation (ChIP) and MIRA analysis of MatDup.dist7 and PatDup.dist7 MEFs provided a panoramic map of reciprocal allele-specific histone modifications and DNA methylation at imprinted genes along distal chromosome 7 and 15. ChIP-chip and MIRA-chip was done to map histone modifications and DNA methylation along distal chr7 in the maternal allele and paternal allele in Matdup.dist7 and Patdup.dist7 MEFs, respectively, using Nimblegen tiling arrays for distal chr7.

ORGANISM(S): Mus musculus

SUBMITTER: Xiwei Wu

PROVIDER: E-GEOD-26891 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Chromosome-wide analysis of parental allele-specific chromatin and DNA methylation.

Singh Purnima P Wu Xiwei X Lee Dong-Hoon DH Li Arthur X AX Rauch Tibor A TA Pfeifer Gerd P GP Mann Jeffrey R JR Szabó Piroska E PE

Molecular and cellular biology 20110214 8

To reveal the extent of domain-wide epigenetic features at imprinted gene clusters, we performed a high-resolution allele-specific chromatin analysis of over 100 megabases along the maternally or paternally duplicated distal chromosome 7 (Chr7) and Chr15 in mouse embryo fibroblasts (MEFs). We found that reciprocal allele-specific features are limited to imprinted genes and their differentially methylated regions (DMRs), whereas broad local enrichment of H3K27me3 (BLOC) is a domain-wide feature a ...[more]

PMID: 21321082

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