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Chromosome wide analysis of parental allele specific chromatin and DNA methylation along mouse distal chr15

ABSTRACT: Imprinted genes are monoallelically expressed according to parental inheritance. The maternally and paternally inherited alleles are distinguished epigenetically by DNA methylation and histone modifications. Chromosome-wide Chromatin immunoprecipitation (ChIP) and MIRA analysis of MatDup.dist7 and PatDup.dist7 MEFs provided a panoramic map of reciprocal allele-specific histone modifications and DNA methylation at imprinted genes along distal chromosome 7 and 15. ChIP-chip and MIRA-chip was done to map histone modifications and DNA methylation along distal chr15 in the maternal allele and paternal allele in Patdup.dist7=MatDup.dist15 and Patdup.dist7=MatDupdis15 MEFs, respectively, using Nimblegen tiling arrays for chr15.

ORGANISM(S): Mus musculus

SUBMITTER: Xiwei Wu

PROVIDER: E-GEOD-26893 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Chromosome-wide analysis of parental allele-specific chromatin and DNA methylation.

Singh Purnima P Wu Xiwei X Lee Dong-Hoon DH Li Arthur X AX Rauch Tibor A TA Pfeifer Gerd P GP Mann Jeffrey R JR Szabó Piroska E PE

Molecular and cellular biology 20110214 8

To reveal the extent of domain-wide epigenetic features at imprinted gene clusters, we performed a high-resolution allele-specific chromatin analysis of over 100 megabases along the maternally or paternally duplicated distal chromosome 7 (Chr7) and Chr15 in mouse embryo fibroblasts (MEFs). We found that reciprocal allele-specific features are limited to imprinted genes and their differentially methylated regions (DMRs), whereas broad local enrichment of H3K27me3 (BLOC) is a domain-wide feature a ...[more]

PMID: 21321082

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