Dataset Information

Quantitative ChIP-MS analysis of histone modifications present on H3K4me1- and H3K4me3-enriched mono-nucleosomes purified from crosslinked IMR-90 cells

ABSTRACT: The INO80 complex is a chromatin remodeler that regulates DNA replication, repair, and transcription. Although the INO80 complex plays a crucial role in various chromatin-associated processes, the mechanism of its recruitment to specific genomic loci is not well understood. Here we used a native ChIP-MS approach to quantitatively profile modifications present on nucleosomes co-purified with INO80 from MNAse-digested HeLa chromatin.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Andrey Tvardovskiy

LAB HEAD: Till Bartke

PROVIDER: PXD042224 | Pride | 2023-12-12

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	ChIP_MS_histones_H3K4me1_repl1.raw	Raw
	ChIP_MS_histones_H3K4me1_repl2.raw	Raw
	ChIP_MS_histones_H3K4me1_repl3.raw	Raw
	ChIP_MS_histones_H3K4me3_repl1.raw	Raw
	ChIP_MS_histones_H3K4me3_repl2.raw	Raw

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Publications

Decoding chromatin states by proteomic profiling of nucleosome readers.

Lukauskas Saulius S Tvardovskiy Andrey A Nguyen Nhuong V NV Stadler Mara M Faull Peter P Ravnsborg Tina T Özdemir Aygenli Bihter B Dornauer Scarlett S Flynn Helen H Lindeboom Rik G H RGH Barth Teresa K TK Brockers Kevin K Hauck Stefanie M SM Vermeulen Michiel M Snijders Ambrosius P AP Müller Christian L CL DiMaggio Peter A PA Jensen Ole N ON Schneider Robert R Bartke Till T

Nature 20240306 8004

DNA and histone modifications combine into characteristic patterns that demarcate functional regions of the genome1,2. While many 'readers' of individual modifications have been described3-5, how chromatin states comprising composite modification signatures, histone variants and internucleosomal linker DNA are interpreted is a major open question. Here we use a multidimensional proteomics strategy to systematically examine the interaction of around 2,000 nuclear proteins wi ...[more]

PMID: 38448585

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