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Clr4/Suv39 and RNA quality control factors cooperate to trigger RNAi and suppress antisense RNA (expression)

ABSTRACT: Expression profile of Schizosaccharomyces pombe genome in ?mlo3, mlo3-A, mlo3-A?h2a.z, ?cid14?h2a.z and mlo3-A?cid14 cells. Expression profile of mutant cells compared to wild-type cells. Agilent 60mer oligonucleotide custom array containing probes spanning large portion of chromosome 2 at 50bp resolution was used to profile expression levels in mutant cells and to compare them to levels in wild-type cells.

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Shiv Grewal

PROVIDER: E-GEOD-26995 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Clr4/Suv39 and RNA quality control factors cooperate to trigger RNAi and suppress antisense RNA.

Zhang Ke K Fischer Tamas T Porter Rebecca L RL Dhakshnamoorthy Jothy J Zofall Martin M Zhou Ming M Veenstra Timothy T Grewal Shiv I S SI

Science (New York, N.Y.) 20110301 6024

Pervasive transcription of eukaryotic genomes generates a plethora of noncoding RNAs. In fission yeast, the heterochromatin factor Clr4/Suv39 methyltransferase facilitates RNA interference (RNAi)-mediated processing of centromeric transcripts into small interfering RNAs (siRNAs). Clr4 also mediates degradation of antisense RNAs at euchromatic loci, but the underlying mechanism has remained elusive. We show that Clr4 and the RNAi effector RITS (RNA-induced transcriptional silencing) interact with ...[more]

PMID: 21436456

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Histone H2A.Z cooperates with RNAi and heterochromatin factors to suppress antisense RNAs

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Clr4/Suv39 and RNA quality control factors cooperate to trigger RNAi and suppress antisense RNA

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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Asf1/HIRA facilitate global histone deacetylation and associate with HP1 to promote nucleosome occupancy at heterochromatic loci (expression)

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