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Clr4/Suv39 and RNA quality control factors cooperate to trigger RNAi and suppress antisense RNA (expression)

ABSTRACT: Expression profile of Schizosaccharomyces pombe genome in ∆mlo3, mlo3-A, mlo3-A∆h2a.z, ∆cid14∆h2a.z and mlo3-A∆cid14 cells. Expression profile of mutant cells compared to wild-type cells.

ORGANISM(S): Schizosaccharomyces pombe

PROVIDER: GSE26995 | GEO | 2011/04/12

SECONDARY ACCESSION(S): PRJNA142035

REPOSITORIES: GEO

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Clr4/Suv39 and RNA quality control factors cooperate to trigger RNAi and suppress antisense RNA (expression)

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Clr4/Suv39 and RNA quality control factors cooperate to trigger RNAi and suppress antisense RNA (ChIP)

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Clr4/Suv39 and RNA quality control factors cooperate to trigger RNAi and suppress antisense RNA (ChIP)

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Clr4/Suv39 and RNA quality control factors cooperate to trigger RNAi and suppress antisense RNA.

Project description:Pervasive transcription of eukaryotic genomes generates a plethora of noncoding RNAs. In fission yeast, the heterochromatin factor Clr4/Suv39 methyltransferase facilitates RNA interference (RNAi)-mediated processing of centromeric transcripts into small interfering RNAs (siRNAs). Clr4 also mediates degradation of antisense RNAs at euchromatic loci, but the underlying mechanism has remained elusive. We show that Clr4 and the RNAi effector RITS (RNA-induced transcriptional silencing) interact with Mlo3, a protein related to mRNA quality control and export factors. Loss of Clr4 impairs RITS interaction with Mlo3, which is required for centromeric siRNA production and antisense suppression. Mlo3 also interacts with the RNA surveillance factor TRAMP, which suppresses antisense RNAs targeted by Clr4 and RNAi. These findings link Clr4 to RNA quality control machinery and suggest a pathway for processing potentially deleterious RNAs through the coordinated actions of RNAi and other RNA processing activities.

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Clr4/Suv39 and RNA quality control factors cooperate to trigger RNAi and suppress antisense RNA

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2011-04-12 | E-GEOD-26999 | biostudies-arrayexpress

Clr4/Suv39 and RNA quality control factors cooperate to trigger RNAi and suppress antisense RNA

Project description:This SuperSeries is composed of the SubSeries listed below.

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Histone H2A.Z cooperates with RNAi and heterochromatin factors to suppress antisense RNAs

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Histone H2A.Z cooperates with RNAi and heterochromatin factors to suppress antisense RNAs

Project description:Expression profile of mutant cells compared to wild-type cells: Expression profile of Schizosaccharomyces pombe genome in ∆pht1, ∆ago1, ∆clr4, rik1, ∆pht1rik1, ∆pht1∆ago1 and ∆pht1∆clr4 cells. Expression profile of Schizosaccharomyces pombe genome in ∆rrp6, ∆cid14, ∆swi6, ∆swr1, ∆set1, ∆pht1∆swi6 and ∆pht1∆set1 cells. Expression profile of Schizosaccharomyces pombe genome in ∆alp13, ∆cph1, ∆set2, clr6-1 and ∆cph1∆pht1 cells. Occupancy profiling: Occupancy profiling of RNA polymerase II, histone variant H2A.Z and ClrC subunit Rik1 in fission yeast Schizosaccharomyces pombe Occupancy profiling of histone variant H2A.Z in ∆msc1 cells. Agilent 60mer oligonucleotide custom array containing probes spanning large portion of chromosome 2 at 50bp resolution was used to profile expression levels in mutant cells and to compare them to levels in wild-type cells. Agilent 60mer array was used to analyze DNA recovered by immunoprecipitation of RNA polymerase II, H2A.Z or Rik1 from asynchronous culture of fission yeast. Agilent 60mer array was used to analyze DNA recovered by immunoprecipitation of histone H2A.Z from asynchronous culture of fission yeast.

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TRAMP-mediated RNA surveillance prevents spurious entry of RNAs into the S. pombe siRNA pathway

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