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TGFB signaling effect on cultured NHLF cells

ABSTRACT: TGFβ activates a signal transduction cascade that results in the microRNAs and genes transcription. The objective of this study is to identify miRNAs which are regulated through TGFB signaling pathway Cultured NHLF cells at 30-50% confluence were treated with 5 ng/mL TGFB1 or DMSO.

ORGANISM(S): Homo sapiens

SUBMITTER: Jadranka Milosevic

PROVIDER: E-GEOD-27156 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Profibrotic role of miR-154 in pulmonary fibrosis.

Milosevic Jadranka J Pandit Kusum K Magister Marcus M Rabinovich Einat E Ellwanger Daniel C DC Yu Guoying G Vuga Louis J LJ Weksler Benny B Benos Panayiotis V PV Gibson Kevin F KF McMillan Michael M Kahn Michael M Kaminski Naftali N

American journal of respiratory cell and molecular biology 20121004 6

In this study, we explored the regulation and the role of up-regulated microRNAs in idiopathic pulmonary fibrosis (IPF), a progressive interstitial lung disease of unknown origin. We analyzed the expression of microRNAs in IPF lungs and identified 43 significantly up-regulated microRNAs. Twenty-four of the 43 increased microRNAs were localized to the chromosome 14q32 microRNA cluster. We validated the increased expression of miR-154, miR-134, miR-299-5p, miR-410, miR-382, miR-409-3p, miR-487b, m ...[more]

PMID: 23043088

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