Unknown,Transcriptomics,Genomics,Proteomics

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Sequencing-based approaches reveal low ambient temperature-responsive and tissue-specific microRNAs in Phalaenopsis orchid


ABSTRACT: We performed four small RNA sequencing for identification and characterization of microRNAs in Phalaenopsis aphrodite subsp. formosana. By comparing the low temperature-treated group with treated group, we concluded four miRNAs - miR156, miR162, miR528 and miR535 - as low temperature-induced miRNAs. In addition, tissue-specific expression of these miRNAs was investigated. The files contain the miRNAs analysis results in each group. Examination of low temperature-treated leaves and two other organs of Phalaenopsis orchid

ORGANISM(S): Phalaenopsis aphrodite subsp. formosana

SUBMITTER: Feng-Ming An 

PROVIDER: E-GEOD-27585 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Sequencing-based approaches reveal low ambient temperature-responsive and tissue-specific microRNAs in phalaenopsis orchid.

An Feng-Ming FM   Hsiao Shuan-Rung SR   Chan Ming-Tsair MT  

PloS one 20110506 5


Plant small RNAs (smRNAs) are short, non-coding RNA molecules that mediate RNA silencing and regulate a group of genes involved in plant development and responses to environmental stimuli. Low temperature is necessary to initiate stalk development in the orchid Phalaenopsis aphrodite subsp. formosana. To identify smRNAs in Phalaenopsis responding to low temperatures, a smRNA profiling analysis using high-throughput sequencing technology was performed. Subsequent bioinformatic analysis was applie  ...[more]

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