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Expression data of human umbilical vein endothelial cells (HUVECs) treated with BMP-6 and BMP-9

ABSTRACT: Smad1/5 are transcription factors that engage in BMP-induced transcription. We determined and analyzed Smad1/5 binding sites by ChIP-sequencing. We used expression microarrays to compare the Smad1/5 binding sites identified by ChIP-seq to BMP-induced gene expressions. HUVECs were treated with BMP for RNA extraction and hybridization on Affymetrix microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Daizo Koinuma

PROVIDER: E-GEOD-27631 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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ChIP-seq reveals cell type-specific binding patterns of BMP-specific Smads and a novel binding motif.

Morikawa Masato M Koinuma Daizo D Tsutsumi Shuichi S Vasilaki Eleftheria E Kanki Yasuharu Y Heldin Carl-Henrik CH Aburatani Hiroyuki H Miyazono Kohei K

Nucleic acids research 20110715 20

Dysregulated bone morphogenetic protein (BMP) signaling in endothelial cells (ECs) and pulmonary arterial smooth muscle cells (PASMCs) are implicated in human genetic disorders. Here, we generated genome-wide maps of Smad1/5 binding sites in ECs and PASMCs. Smad1/5 preferentially bound to the region outside the promoter of known genes, and the binding was associated with target gene upregulation. Cell-selective Smad1/5 binding patterns appear to be determined mostly by cell-specific differences ...[more]

PMID: 21764776

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