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Expression data of human pulmonary arterial smooth muscle cells (PASMCs) treated with BMP-4

ABSTRACT: Smad1/5 are transcription factors that engage in BMP-induced transcription. We determined and analyzed Smad1/5 binding sites by ChIP-sequencing. We used expression microarrays to compare the Smad1/5 binding sites identified by ChIP-seq to BMP-induced gene expressions. PASMCs were treated with 50 ng/ml BMP-4 for 0, 2, or 24 hrs. RNA was extracted and hybridized on Affymetrix microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Daizo Koinuma

PROVIDER: E-GEOD-28847 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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ChIP-seq reveals cell type-specific binding patterns of BMP-specific Smads and a novel binding motif.

Morikawa Masato M Koinuma Daizo D Tsutsumi Shuichi S Vasilaki Eleftheria E Kanki Yasuharu Y Heldin Carl-Henrik CH Aburatani Hiroyuki H Miyazono Kohei K

Nucleic acids research 20110715 20

Dysregulated bone morphogenetic protein (BMP) signaling in endothelial cells (ECs) and pulmonary arterial smooth muscle cells (PASMCs) are implicated in human genetic disorders. Here, we generated genome-wide maps of Smad1/5 binding sites in ECs and PASMCs. Smad1/5 preferentially bound to the region outside the promoter of known genes, and the binding was associated with target gene upregulation. Cell-selective Smad1/5 binding patterns appear to be determined mostly by cell-specific differences ...[more]

PMID: 21764776

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Expression data of human umbilical vein endothelial cells (HUVECs) treated with BMP-6 and BMP-9

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Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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ZSWIM4 regulates early embryonic patterning and BMP signaling pathway by promoting nuclear Smad1 degradation

Project description:The dorsoventral gradient of BMP signaling plays an essential role in embryonic patterning, with high BMP signal activating ventral-lateral mesoderm markers directly, and low BMP signal inducing neural tissues. The Zinc finger SWIM domain-containing protein 4 (zswim4) is expressed in the dorsal blastopore lip at the onset of Xenopus gastrula and then enriched at the forming neuroectoderm at mid-gastrula stages. Overexpression of zswim4 in Xenopus embryos causes inhibition of the anterior axis and shortened, curved body, and knockdown or knockout of zswim4 disturbed embryonic body axis formation and head development. The expression of ventral-lateral mesoderm marker genes was reduced after zswim4 overexpression and increased in embryos with zswim4 knockdown. Neural marker genes were repressed in zswim4 morphant. Mechanistically Zswim4 attenuates BMP signal through reducing protein stability of Smad1 in both Xenopus embryos and HEK293T cells. Zswim4 interacts with Smad1 and promotes ubiquitination of Smad1 in HEK293T cells. To identify the interaction partner of Zswim4 in regulating Smad1 stability, we performed SILAC based IP in HEK293T cells, and the precipitates were analyzed by Mass Spectrometry.

2024-05-22 | PXD035207 | Pride

Homo sapiens

Project description:SMAD1/5 binding regions of human pulmonary arterial smooth muscle cells (PASMCs) treated with BMP

| PRJNA143115 | ENA

SMAD1/5 binding regions of human pulmonary arterial smooth muscle cells (PASMCs) treated with BMP

2011-07-16 | GSE28848 | GEO

Expression data of human pulmonary arterial smooth muscle cells (PASMCs) treated with BMP-4

2011-07-16 | GSE28847 | GEO

Expression data of human umbilical vein endothelial cells (HUVECs) treated with BMP-6 and BMP-9

2011-07-16 | GSE27631 | GEO

Transcriptome of MEFs with BMP2 and DNA damage reagents

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