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Transcription profiling of Drosophila melanogaster BAM over expressing germline stem cells vs controls

ABSTRACT: Gene expression is tested for an association with GSCs by comparing expression levels in ovary tips of c587-GAL4; hs-bam UAS-dpp females at 0 hr, 20 hr and 50 hr after heat shock ; results of two independent experiments are given as expt 1 and expt 2 Experiment Overall Design: 0 hr ovary tips contain many GSCs Experiment Overall Design: 20 hr hs-bam has induced differentiation of all GSCs Experiment Overall Design: 50 hr Bam has turned over and cystocytes have reverted to become GSCs again Experiment Overall Design: see Kai et al. (2005) Dev. Biol, in press.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Allan Spradling

PROVIDER: E-GEOD-2784 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The expression profile of purified Drosophila germline stem cells.

Kai Toshie T Williams Dianne D Spradling Allan C AC

Developmental biology 20050701 2

We developed a method to highly purify germline stem cells (GSCs) from the Drosophila ovary, one of the best understood types of adult stem cell. GSCs express variant isoforms of general transcriptional components, translation initiation factors, and several variant ribosomal proteins, including RpL22, a protein enriched in several mammalian stem cells. These novel isoforms may help regulate stem cell gene expression because a reversion assay indicated that at least four were specific for GSCs. ...[more]

PMID: 15927177

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GSC differentiation-reversion test

Project description:gene expression is tested for an association with GSCs by comparing expression levels in ovary tips of c587-GAL4; hs-bam UAS-dpp females at 0 hr, 20 hr and 50 hr after heat shock ; results of two independent experiments are given as expt 1 and expt 2 Keywords: time course, heat shock, bam overexpression

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