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The effects of the 5' pocket motif of Dicer on miRNA biogenesis

ABSTRACT: A hallmark of RNA silencing is a class of ~22 nt RNAs which are processed from dsRNA precursor by Dicer. Accurate processing by Dicer is critical for the functionality of microRNAs (miRNAs). According to the current model, Dicer measures the length by anchoring the 3' overhang of the dsRNA terminus. Here we find that human Dicer binds to the 5' end of RNA and utilizes the 5' end as an additional reference point for cleavage site selection (5' counting rule). We further identify a novel motif (5'-pocket) in Dicer, which recognizes the 5' end of RNA. By analyzing miRNA population from 5'-pocket mutant Dicer expressing Dicer-null mES, we provide that the 5' pocket is significant for Dicer processing in vivo. Examination of small RNA profiles from Dicer-null mouse embryonic stem cells transfected with either wild-type or 5' pocket mutant Dice expression plasmids.

ORGANISM(S): Mus musculus

SUBMITTER: Hyeshik Chang

PROVIDER: E-GEOD-27903 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Dicer recognizes the 5' end of RNA for efficient and accurate processing.

Park Jong-Eun JE Heo Inha I Tian Yuan Y Simanshu Dhirendra K DK Chang Hyeshik H Jee David D Patel Dinshaw J DJ Kim V Narry VN

Nature 20110713 7355

A hallmark of RNA silencing is a class of approximately 22-nucleotide RNAs that are processed from double-stranded RNA precursors by Dicer. Accurate processing by Dicer is crucial for the functionality of microRNAs (miRNAs). The current model posits that Dicer selects cleavage sites by measuring a set distance from the 3' overhang of the double-stranded RNA terminus. Here we report that human Dicer anchors not only the 3' end but also the 5' end, with the cleavage site determined mainly by the d ...[more]

PMID: 21753850

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