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Effect of fasting on human skeletal muscle mRNA levels

ABSTRACT: The goal of these studies was to determine the effects of fasting on skeletal muscle mRNA levels in healthy human subjects. Seven healthy adult human subjects fasted for 40 hours and then a muscle biopsy (fasting sample) was obtained from the vastus lateralis muscle. Immediately after the first muscle biospy, subjects then ate a mixed meal. Six hours after the first muscle biopsy, a second muscle biopsy (fed sample) was obtained from the contralateral vastus lateralis muscle. In each subject, mRNA levels under fasting conditions were normalized to mRNA levels under fed conditions, which were set at 1.

ORGANISM(S): Homo sapiens

SUBMITTER: Christopher Adams

PROVIDER: E-GEOD-28016 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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mRNA expression signatures of human skeletal muscle atrophy identify a natural compound that increases muscle mass.

Kunkel Steven D SD Suneja Manish M Ebert Scott M SM Bongers Kale S KS Fox Daniel K DK Malmberg Sharon E SE Alipour Fariborz F Shields Richard K RK Adams Christopher M CM

Cell metabolism 20110601 6

Skeletal muscle atrophy is a common and debilitating condition that lacks a pharmacologic therapy. To develop a potential therapy, we identified 63 mRNAs that were regulated by fasting in both human and mouse muscle, and 29 mRNAs that were regulated by both fasting and spinal cord injury in human muscle. We used these two unbiased mRNA expression signatures of muscle atrophy to query the Connectivity Map, which singled out ursolic acid as a compound whose signature was opposite to those of atrop ...[more]

PMID: 21641545

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