Unknown,Transcriptomics,Genomics,Proteomics

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Influence of low and high force muscle activity on paralyzed muscle gene expression


ABSTRACT: We used a novel approach to study the acute effect of two frequencies of stimulation (20 Hz and 5 Hz; high and low force, respectively) on gene regulation in people with chronic paralysis. Three hours after the completion of the electrical stimulation protocol (5 Hz or 20 Hz), we sampled the vastus lateralis muscle and examined genes involved with metabolic transcription, glycolysis, oxidative phosphorylation, and mitochondria remodelling. We discovered that the 5 Hz stimulation, despite generating a lower overall force, induced a 5-6 fold increase (p<0.05) in key metabolic transcription factors including PGC-1?, NR4A3, and ABRA. Neither protocol showed a robust regulation of genes for glycolysis, oxidative phosphorylation, and mitochondria remodelling. We analyzed skeletal muscle using the Affymetrix Human Exon 1.0 ST platform. Array data was processed by Partek Genomic Suites.

ORGANISM(S): Homo sapiens

SUBMITTER: Richard Shields 

PROVIDER: E-GEOD-64683 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Low-frequency stimulation regulates metabolic gene expression in paralyzed muscle.

Petrie Michael M   Suneja Manish M   Shields Richard K RK  

Journal of applied physiology (Bethesda, Md. : 1985) 20150129 6


The altered metabolic state after a spinal cord injury compromises systemic glucose regulation. Skeletal muscle atrophies and transforms into fast, glycolytic, and insulin-resistant tissue. Osteoporosis is common after spinal cord injury and limits the ability to exercise paralyzed muscle. We used a novel approach to study the acute effect of two frequencies of stimulation (20 and 5 Hz) on muscle fatigue and gene regulation in people with chronic paralysis. Twelve subjects with chronic (>1 yr) a  ...[more]

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