Unknown,Transcriptomics,Genomics,Proteomics

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MRNA-Seq of whole flies from Drosophila

ABSTRACT: We sequenced mRNA from whole flies of females and male of Drosophila melanogaster, D. simulans, D. yakuba, D. ananassae, D. pseudobscura, D. mojavensis, and D. virilis as part of the modENCODE project to validate novel gene models found in D. melanogaster, identify genes differentially expressed between the sexes and sex-specific alternative splicing events, and examine the evolution of gene expression. Comparison of expresssion profiles in female and male whole flies. These are the same samples used to generate the array data found in GSE6640.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Brian Oliver 

PROVIDER: E-GEOD-28078 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The developmental transcriptome of Drosophila melanogaster.

Graveley Brenton R BR   Brooks Angela N AN   Carlson Joseph W JW   Duff Michael O MO   Landolin Jane M JM   Yang Li L   Artieri Carlo G CG   van Baren Marijke J MJ   Boley Nathan N   Booth Benjamin W BW   Brown James B JB   Cherbas Lucy L   Davis Carrie A CA   Dobin Alex A   Li Renhua R   Lin Wei W   Malone John H JH   Mattiuzzo Nicolas R NR   Miller David D   Sturgill David D   Tuch Brian B BB   Zaleski Chris C   Zhang Dayu D   Blanchette Marco M   Dudoit Sandrine S   Eads Brian B   Green Richard E RE   Hammonds Ann A   Jiang Lichun L   Kapranov Phil P   Langton Laura L   Perrimon Norbert N   Sandler Jeremy E JE   Wan Kenneth H KH   Willingham Aarron A   Zhang Yu Y   Zou Yi Y   Andrews Justen J   Bickel Peter J PJ   Brenner Steven E SE   Brent Michael R MR   Cherbas Peter P   Gingeras Thomas R TR   Hoskins Roger A RA   Kaufman Thomas C TC   Oliver Brian B   Celniker Susan E SE  

Nature 20101222 7339

Drosophila melanogaster is one of the most well studied genetic model organisms; nonetheless, its genome still contains unannotated coding and non-coding genes, transcripts, exons and RNA editing sites. Full discovery and annotation are pre-requisites for understanding how the regulation of transcription, splicing and RNA editing directs the development of this complex organism. Here we used RNA-Seq, tiling microarrays and cDNA sequencing to explore the transcriptome in 30 distinct developmental  ...[more]

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