Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

58 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Discovery of Gene Expression Signature in the Overexpression of miR-145 in U87 glioma cell.

ABSTRACT: We have employed whole RNA microarray expression profiling as a discovery platform to identify genes regulated by overexpression of miR-145 in U87 glioma cell. Lentivirus containing miR-145 coding sequence was infected to U87 cell to make U87 overexpressing miR-145. We did genome microarray between U87 and U87 overexpressing miR-145. Total RNAs from U87 cell or U87 overexpressing miR-145 were extracted. Whole RNA microarray expression profiling was performed between them.

ORGANISM(S): Homo sapiens

SUBMITTER: Sang-Jin Lee

PROVIDER: E-GEOD-28121 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Discovery of Gene Expression Signature in the Overexpression of miR-145 in U87 glioma cell.

Project description:We have employed whole RNA microarray expression profiling as a discovery platform to identify genes regulated by overexpression of miR-145 in U87 glioma cell. Lentivirus containing miR-145 coding sequence was infected to U87 cell to make U87 overexpressing miR-145. We did genome microarray between U87 and U87 overexpressing miR-145.

2011-03-25 | GSE28121 | GEO

Gene expression profiles of U87 cells after transfection with hsa-miR-145-5p and 31-5p

Project description:We analyzed the expression profiles of hsa-miR-145-5p or hsa-miR-31-5p-targeting genes relating to invasion or migration after co-overexpression of hsa-miR-145-5p and 31-5p Gene expression profiles of U87 cells after co-transfection with hsa-miR-145-5p and 31-5p mimics, and U87 cells after transfection miR mimic negative control

2016-06-23 | E-GEOD-83643 | biostudies-arrayexpress

Gene profiles during vestibular compensation in unilateral labyrinthectomized rats

Project description:Microarray analysis was used to examine the expression of genes upregulated or downregulated in the ipsilateral vestibular nucleus at 1 and 7 days following unilateral labyrinthectomy. Changes in gene expression during the chronic phase of vestibular compensation following unilateral labyrinthectomy in rats Three conditioned experiment: nl:control with sham operation, 1day:1day after labyrninthectomy, 7day:7day after labyrinthectomy

2011-11-16 | E-GEOD-32437 | biostudies-arrayexpress

Comparable mRNA expression of pluripotency-related genes in SNUhES3 cells cultured on autofeeder or xenofeeder system

Project description:I developed a new culture system for hES cells; this system does not require supplementation with bFGF to obtain hES cells that are suitable for tissue engineering and regenerative medicine. This culture system employed mesenchymal stem cells derived from hES cells (hESC-MSCs) as autologous human feeder cells in the absence of bFGF. For pluripotency-related gene expression profiling, a cDNA microarray analysis was performed SNUhES3 cultured on the autofeeder hESC-MSCs layer maintained the undifferentiated state for 30 passages in a manner similar to the SNUhES3 cells on xenofeeder STO cell layer. To compare the pluripotency-related genes in SNUhES3 cells cultured on autofeeder or xenofeeder system, I used agilent one-color array. Two independant experiment performed.

2011-02-02 | E-GEOD-26993 | biostudies-arrayexpress

Identification of miR-145 targets using SILAC

Project description:miR-145 is downregulated in multiple cancers. The introduction of miR-145 could alleviate the tumor burden in the pancreatic cancer mouse model. However, how miR-145 mediates the tumor suppression is still an open question. In this study, we aimed to identify the targets of miR-145 using a SILAC approach.

2014-10-28 | PXD001260 | Pride

Microarray expression profiling of mRNAs using RNA extracted from miR-151a-overexpressing and miR-NC overexpressing U87 glioma cells.

Project description:Using microarray technology, we aimed to produce a list of genes that are downregulated in miR-151a-overexpressing U87 glioma cells in comparison with these overexpressing miR-NC.

2017-03-15 | GSE96606 | GEO

miR-145 transfected PC3 cells

Project description:It is aimed to reveal overall trancriptional change in prostate cancer PC3 cells after ectopic expression of miR-145 Pre-mir-145 transfected PC3 cells were collected at 8, 16 and 24 hours after transfection and control untrasfected PC3 cells were used.

2014-06-15 | E-GEOD-58295 | biostudies-arrayexpress

Expression analysis of Hsa-miR-145-5p overexpressed laryngeal squamous carcinoma cell line TU177

Project description:Investigation of whole genome gene expression level changes in laryngeal squamous carcinoma cell line TU177 in response to overexpressed miR-145-5p. Differentially expressed protein-coding genes in the human laryngeal squamous carcinoma cells TU177 overexpressing miR-145-5p were identified by microarray analysis.

2017-12-31 | GSE92678 | GEO

MicroRNA expression profiling in laryngeal squamous cell carcinoma (LSCC)

Project description:In this study, we aimed to explore the role of miR-145, which is downregulated in LSCC, on cancer stem cell potency of laryngeal cancer cells. Using microRNA expression profiling and experimental follow-up studies, we demonstrated the regulatory role of miR-145 in stem cell characteristics of Hep-2 cells. Based on these results, we propose that mir-145 might carry crucial roles in LSCC tumorigenesis, prognosis, metastasis, chemoresistance and recurrence through regulating stem cell properties of tumor cells. LSCC tissue samples and normal tissues were profiled for microRNA expression; cancer tissue profiles were compared with normal tissue profiles.

2015-11-10 | E-GEOD-70289 | biostudies-arrayexpress

Comparison between wild type E. coli and adhE (alcohol dehydrogenase) mutants, grown under anaerobic condition

Project description:To characterize anaerobic stress-induced expression in adhE mutants, the microarray experiment was performed with two serotypes of wild type E. coli and their adhE mutants: (1) K-12 strain, wild type; (2) B strain, wild type; (3) BW25113, adhE mutants; (4) BL21(DE3), adhE mutants. Under anaerobic growth condition in glucose-containing complex medium, the wild type strains 1 and 2 grew well whereas the mutant strains 3 and 4 experienced anaerobic stress and grew after 24 hours and 48 hours, respectively. For each of the four strains, RNA sampling was done after six hours of growth under anaerobic condition. Only one replicate was obtained for each strain.

2014-09-26 | E-GEOD-46455 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data