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Genome-wide analysis of REST knockdown responsive gene expression in mouse ES cells

ABSTRACT: Analysis of gene expression profiling upon REST shRNA knockdown in mouse ES cells for 72 hours, Total RNA obtained from pSuper vector control and REST shRNA treatment for 72 hours using Invitrogen RNA extraction kit.

ORGANISM(S): Mus musculus

SUBMITTER: hongbing yu

PROVIDER: E-GEOD-28141 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Coassembly of REST and its cofactors at sites of gene repression in embryonic stem cells.

Yu Hong-Bing HB Johnson Rory R Kunarso Galih G Stanton Lawrence W LW

Genome research 20110601 8

The differentiation of pluripotent embryonic stem cells is regulated by networks of activating and repressing transcription factors that orchestrate determinate patterns of gene expression. With the recent mapping of target sites for many transcription factors, it has been a conundrum that so few of the genes directly targeted by these factors are transcriptionally responsive to the binding of that factor. To address this, we generated genome-wide maps of the transcriptional repressor REST and f ...[more]

PMID: 21632747

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